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Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zeb...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786895/ https://www.ncbi.nlm.nih.gov/pubmed/36555564 http://dx.doi.org/10.3390/ijms232415921 |
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author | Lee, Hung-Chieh Lai, Wei-Lin Lin, Cheng-Yung Zeng, Chih-Wei Sheu, Jin-Chuan Chou, Tze-Bin Tsai, Huai-Jen |
author_facet | Lee, Hung-Chieh Lai, Wei-Lin Lin, Cheng-Yung Zeng, Chih-Wei Sheu, Jin-Chuan Chou, Tze-Bin Tsai, Huai-Jen |
author_sort | Lee, Hung-Chieh |
collection | PubMed |
description | After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos. |
format | Online Article Text |
id | pubmed-9786895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97868952022-12-24 Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos Lee, Hung-Chieh Lai, Wei-Lin Lin, Cheng-Yung Zeng, Chih-Wei Sheu, Jin-Chuan Chou, Tze-Bin Tsai, Huai-Jen Int J Mol Sci Article After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos. MDPI 2022-12-14 /pmc/articles/PMC9786895/ /pubmed/36555564 http://dx.doi.org/10.3390/ijms232415921 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Hung-Chieh Lai, Wei-Lin Lin, Cheng-Yung Zeng, Chih-Wei Sheu, Jin-Chuan Chou, Tze-Bin Tsai, Huai-Jen Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title | Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title_full | Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title_fullStr | Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title_full_unstemmed | Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title_short | Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos |
title_sort | anp32a promotes neuronal regeneration after spinal cord injury of zebrafish embryos |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786895/ https://www.ncbi.nlm.nih.gov/pubmed/36555564 http://dx.doi.org/10.3390/ijms232415921 |
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