Cargando…

Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos

After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zeb...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hung-Chieh, Lai, Wei-Lin, Lin, Cheng-Yung, Zeng, Chih-Wei, Sheu, Jin-Chuan, Chou, Tze-Bin, Tsai, Huai-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786895/
https://www.ncbi.nlm.nih.gov/pubmed/36555564
http://dx.doi.org/10.3390/ijms232415921
_version_ 1784858397582032896
author Lee, Hung-Chieh
Lai, Wei-Lin
Lin, Cheng-Yung
Zeng, Chih-Wei
Sheu, Jin-Chuan
Chou, Tze-Bin
Tsai, Huai-Jen
author_facet Lee, Hung-Chieh
Lai, Wei-Lin
Lin, Cheng-Yung
Zeng, Chih-Wei
Sheu, Jin-Chuan
Chou, Tze-Bin
Tsai, Huai-Jen
author_sort Lee, Hung-Chieh
collection PubMed
description After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos.
format Online
Article
Text
id pubmed-9786895
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97868952022-12-24 Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos Lee, Hung-Chieh Lai, Wei-Lin Lin, Cheng-Yung Zeng, Chih-Wei Sheu, Jin-Chuan Chou, Tze-Bin Tsai, Huai-Jen Int J Mol Sci Article After spinal cord injury (SCI) in mammals, neuronal regeneration is limited; in contrast, such regeneration occurs quickly in zebrafish. Member A of the acidic nuclear phosphoprotein 32 (ANP32a) family is involved in neuronal development, but its function is controversial, and its involvement in zebrafish SCI remains unknown. To determine the role of zebrafish ANP32a in the neuronal regeneration of SCI embryos, we microinjected ANP32a mRNA into embryos from zebrafish transgenic line Tg(mnx1:GFP) prior to SCI. Compared to control SCI embryos, the results showed that the regeneration of spinal cord and resumption of swimming capability were promoted by the overexpression of ANP32a mRNA but reduced by its knockdown. We next combined fluorescence-activated cell sorting with immunochemical staining of anti-GFAP and immunofluorescence staining against anti-PH3 on Tg(gfap:GFP) SCI embryos. The results showed that ANP32a promoted the proliferation and cell number of radial glial cells at the injury epicenter at 24 h post-injury (hpi). Moreover, when we applied BrdU labeling to SCI embryos derived from crossing the Tg(gfap:GFP) and Tg(mnx1:TagRFP) lines, we found that both radial glial cells and motor neurons had proliferated, along with their increased cell numbers in Anp32a-overexpression SCI-embryos. On this basis, we conclude that ANP32a plays a positive role in the regeneration of zebrafish SCI embryos. MDPI 2022-12-14 /pmc/articles/PMC9786895/ /pubmed/36555564 http://dx.doi.org/10.3390/ijms232415921 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Hung-Chieh
Lai, Wei-Lin
Lin, Cheng-Yung
Zeng, Chih-Wei
Sheu, Jin-Chuan
Chou, Tze-Bin
Tsai, Huai-Jen
Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title_full Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title_fullStr Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title_full_unstemmed Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title_short Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos
title_sort anp32a promotes neuronal regeneration after spinal cord injury of zebrafish embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786895/
https://www.ncbi.nlm.nih.gov/pubmed/36555564
http://dx.doi.org/10.3390/ijms232415921
work_keys_str_mv AT leehungchieh anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT laiweilin anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT linchengyung anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT zengchihwei anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT sheujinchuan anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT choutzebin anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos
AT tsaihuaijen anp32apromotesneuronalregenerationafterspinalcordinjuryofzebrafishembryos