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Recent advances in isobaric labeling and applications in quantitative proteomics

Mass spectrometry (MS) has emerged at the forefront of quantitative proteomic techniques. Liquid chromatography‐mass spectrometry (LC‐MS) can be used to determine abundances of proteins and peptides in complex biological samples. Several methods have been developed and adapted for accurate quantific...

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Autores principales: Sivanich, Michael K., Gu, Ting‐Jia, Tabang, Dylan Nicholas, Li, Lingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787039/
https://www.ncbi.nlm.nih.gov/pubmed/35687565
http://dx.doi.org/10.1002/pmic.202100256
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author Sivanich, Michael K.
Gu, Ting‐Jia
Tabang, Dylan Nicholas
Li, Lingjun
author_facet Sivanich, Michael K.
Gu, Ting‐Jia
Tabang, Dylan Nicholas
Li, Lingjun
author_sort Sivanich, Michael K.
collection PubMed
description Mass spectrometry (MS) has emerged at the forefront of quantitative proteomic techniques. Liquid chromatography‐mass spectrometry (LC‐MS) can be used to determine abundances of proteins and peptides in complex biological samples. Several methods have been developed and adapted for accurate quantification based on chemical isotopic labeling. Among various chemical isotopic labeling techniques, isobaric tagging approaches rely on the analysis of peptides from MS2‐based quantification rather than MS1‐based quantification. In this review, we will provide an overview of several isobaric tags along with some recent developments including complementary ion tags, improvements in sensitive quantitation of analytes with lower abundance, strategies to increase multiplexing capabilities, and targeted analysis strategies. We will also discuss limitations of isobaric tags and approaches to alleviate these restrictions through bioinformatic tools and data acquisition methods. This review will highlight several applications of isobaric tags, including biomarker discovery and validation, thermal proteome profiling, cross‐linking for structural investigations, single‐cell analysis, top‐down proteomics, along with applications to different molecules including neuropeptides, glycans, metabolites, and lipids, while providing considerations and evaluations to each application.
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spelling pubmed-97870392022-12-27 Recent advances in isobaric labeling and applications in quantitative proteomics Sivanich, Michael K. Gu, Ting‐Jia Tabang, Dylan Nicholas Li, Lingjun Proteomics Review Mass spectrometry (MS) has emerged at the forefront of quantitative proteomic techniques. Liquid chromatography‐mass spectrometry (LC‐MS) can be used to determine abundances of proteins and peptides in complex biological samples. Several methods have been developed and adapted for accurate quantification based on chemical isotopic labeling. Among various chemical isotopic labeling techniques, isobaric tagging approaches rely on the analysis of peptides from MS2‐based quantification rather than MS1‐based quantification. In this review, we will provide an overview of several isobaric tags along with some recent developments including complementary ion tags, improvements in sensitive quantitation of analytes with lower abundance, strategies to increase multiplexing capabilities, and targeted analysis strategies. We will also discuss limitations of isobaric tags and approaches to alleviate these restrictions through bioinformatic tools and data acquisition methods. This review will highlight several applications of isobaric tags, including biomarker discovery and validation, thermal proteome profiling, cross‐linking for structural investigations, single‐cell analysis, top‐down proteomics, along with applications to different molecules including neuropeptides, glycans, metabolites, and lipids, while providing considerations and evaluations to each application. John Wiley and Sons Inc. 2022-06-22 2022-10 /pmc/articles/PMC9787039/ /pubmed/35687565 http://dx.doi.org/10.1002/pmic.202100256 Text en © 2022 The Authors. Proteomics published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Review
Sivanich, Michael K.
Gu, Ting‐Jia
Tabang, Dylan Nicholas
Li, Lingjun
Recent advances in isobaric labeling and applications in quantitative proteomics
title Recent advances in isobaric labeling and applications in quantitative proteomics
title_full Recent advances in isobaric labeling and applications in quantitative proteomics
title_fullStr Recent advances in isobaric labeling and applications in quantitative proteomics
title_full_unstemmed Recent advances in isobaric labeling and applications in quantitative proteomics
title_short Recent advances in isobaric labeling and applications in quantitative proteomics
title_sort recent advances in isobaric labeling and applications in quantitative proteomics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787039/
https://www.ncbi.nlm.nih.gov/pubmed/35687565
http://dx.doi.org/10.1002/pmic.202100256
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