Cargando…
Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2
Within the successive waves that occurred during the SARS-CoV-2 pandemic, recommendations arose to test symptomatic and contact subjects by using rapid antigen devices directed against the viral nucleocapsid protein with the aim to isolate contagious patients without delay. The objective of this stu...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787475/ https://www.ncbi.nlm.nih.gov/pubmed/36560632 http://dx.doi.org/10.3390/v14122628 |
_version_ | 1784858520777129984 |
---|---|
author | Pillet, Sylvie Courtieux, Julien Gonzalo, Sylvie Bechri, Issam Bourlet, Thomas Valette, Martine Bal, Antonin Pozzetto, Bruno |
author_facet | Pillet, Sylvie Courtieux, Julien Gonzalo, Sylvie Bechri, Issam Bourlet, Thomas Valette, Martine Bal, Antonin Pozzetto, Bruno |
author_sort | Pillet, Sylvie |
collection | PubMed |
description | Within the successive waves that occurred during the SARS-CoV-2 pandemic, recommendations arose to test symptomatic and contact subjects by using rapid antigen devices directed against the viral nucleocapsid protein with the aim to isolate contagious patients without delay. The objective of this study was to evaluate the ability of four rapid lateral-flow tests (RLFT) that were commercially available on the French market in 2022 to recognize various strains of SARS-CoV-2. Series of five-fold dilutions of seven viral suspensions belonging to different lineages of SARS-CoV-2 (19A, 20A, Alpha, Beta, Gamma, Delta and Omicron) were used to evaluate the analytical sensitivity of four commercially available RLFTs (manufacturers: Abbott, AAZ, Becton-Dickinson and Biospeedia). Cell culture and quantitative RT-PCR were used as references. Excellent correlations were observed for each lineage strain between the viral titer obtained via cell culture and the number of RNA copies measured by quantitative RT-PCR. Although the four tests were able to recognize all the tested variants, significant differences in terms of sensitivity were observed between the four RLFTs. Despite the limitation represented by the small number of devices and clinical isolates that were tested, this study contributed by rapidly comparing the sensitivity of SARS-CoV-2 RLFTs in the Omicron era. |
format | Online Article Text |
id | pubmed-9787475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97874752022-12-24 Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 Pillet, Sylvie Courtieux, Julien Gonzalo, Sylvie Bechri, Issam Bourlet, Thomas Valette, Martine Bal, Antonin Pozzetto, Bruno Viruses Communication Within the successive waves that occurred during the SARS-CoV-2 pandemic, recommendations arose to test symptomatic and contact subjects by using rapid antigen devices directed against the viral nucleocapsid protein with the aim to isolate contagious patients without delay. The objective of this study was to evaluate the ability of four rapid lateral-flow tests (RLFT) that were commercially available on the French market in 2022 to recognize various strains of SARS-CoV-2. Series of five-fold dilutions of seven viral suspensions belonging to different lineages of SARS-CoV-2 (19A, 20A, Alpha, Beta, Gamma, Delta and Omicron) were used to evaluate the analytical sensitivity of four commercially available RLFTs (manufacturers: Abbott, AAZ, Becton-Dickinson and Biospeedia). Cell culture and quantitative RT-PCR were used as references. Excellent correlations were observed for each lineage strain between the viral titer obtained via cell culture and the number of RNA copies measured by quantitative RT-PCR. Although the four tests were able to recognize all the tested variants, significant differences in terms of sensitivity were observed between the four RLFTs. Despite the limitation represented by the small number of devices and clinical isolates that were tested, this study contributed by rapidly comparing the sensitivity of SARS-CoV-2 RLFTs in the Omicron era. MDPI 2022-11-25 /pmc/articles/PMC9787475/ /pubmed/36560632 http://dx.doi.org/10.3390/v14122628 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Pillet, Sylvie Courtieux, Julien Gonzalo, Sylvie Bechri, Issam Bourlet, Thomas Valette, Martine Bal, Antonin Pozzetto, Bruno Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title | Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title_full | Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title_fullStr | Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title_full_unstemmed | Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title_short | Evaluation of Rapid Lateral-Flow Tests Directed against the SARS-CoV-2 Nucleoprotein Using Viral Suspensions Belonging to Different Lineages of SARS-CoV-2 |
title_sort | evaluation of rapid lateral-flow tests directed against the sars-cov-2 nucleoprotein using viral suspensions belonging to different lineages of sars-cov-2 |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787475/ https://www.ncbi.nlm.nih.gov/pubmed/36560632 http://dx.doi.org/10.3390/v14122628 |
work_keys_str_mv | AT pilletsylvie evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT courtieuxjulien evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT gonzalosylvie evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT bechriissam evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT bourletthomas evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT valettemartine evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT balantonin evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 AT pozzettobruno evaluationofrapidlateralflowtestsdirectedagainstthesarscov2nucleoproteinusingviralsuspensionsbelongingtodifferentlineagesofsarscov2 |