The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion

Photoswitchable fluorescent proteins (FPs) have become indispensable tools for studying life sciences. mSAASoti FP, a biphotochromic FP, is an important representative of this protein family. We created a series of mSAASoti mutants in order to obtain fast photoswitchable variants with high brightnes...

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Detalles Bibliográficos
Autores principales: Gavshina, Alexandra V., Solovyev, Ilya D., Savitsky, Alexander P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787662/
https://www.ncbi.nlm.nih.gov/pubmed/36555699
http://dx.doi.org/10.3390/ijms232416058
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author Gavshina, Alexandra V.
Solovyev, Ilya D.
Savitsky, Alexander P.
author_facet Gavshina, Alexandra V.
Solovyev, Ilya D.
Savitsky, Alexander P.
author_sort Gavshina, Alexandra V.
collection PubMed
description Photoswitchable fluorescent proteins (FPs) have become indispensable tools for studying life sciences. mSAASoti FP, a biphotochromic FP, is an important representative of this protein family. We created a series of mSAASoti mutants in order to obtain fast photoswitchable variants with high brightness. K145P mSAASoti has the highest molar extinction coefficient of all SAASoti mutants studied; C21N/K145P/M163A switches to the dark state 36 times faster than mSAASoti, but it lost its ability to undergo green-to-red photoconversion. Finally, the C21N/K145P/F177S and C21N/K145P/M163A/F177S variants demonstrated a high photoswitching rate between both green and red forms.
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spelling pubmed-97876622022-12-24 The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion Gavshina, Alexandra V. Solovyev, Ilya D. Savitsky, Alexander P. Int J Mol Sci Communication Photoswitchable fluorescent proteins (FPs) have become indispensable tools for studying life sciences. mSAASoti FP, a biphotochromic FP, is an important representative of this protein family. We created a series of mSAASoti mutants in order to obtain fast photoswitchable variants with high brightness. K145P mSAASoti has the highest molar extinction coefficient of all SAASoti mutants studied; C21N/K145P/M163A switches to the dark state 36 times faster than mSAASoti, but it lost its ability to undergo green-to-red photoconversion. Finally, the C21N/K145P/F177S and C21N/K145P/M163A/F177S variants demonstrated a high photoswitching rate between both green and red forms. MDPI 2022-12-16 /pmc/articles/PMC9787662/ /pubmed/36555699 http://dx.doi.org/10.3390/ijms232416058 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gavshina, Alexandra V.
Solovyev, Ilya D.
Savitsky, Alexander P.
The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title_full The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title_fullStr The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title_full_unstemmed The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title_short The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion
title_sort role of the 145 residue in photochemical properties of the biphotochromic protein msaasoti: brightness versus photoconversion
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787662/
https://www.ncbi.nlm.nih.gov/pubmed/36555699
http://dx.doi.org/10.3390/ijms232416058
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