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Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats

BACKGROUND: Persistent visceral hypersensitivity is a key component of functional and inflammatory gastrointestinal diseases. Current animal models fail to fully reproduce the characteristics of visceral pain in humans, particularly as it relates to persistent hypersensitivity. This work explores th...

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Autores principales: López‐Estévez, Sergio, López‐Torrellardona, Josep Manuel, Parera, Marc, Martínez, Vicente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787759/
https://www.ncbi.nlm.nih.gov/pubmed/36239298
http://dx.doi.org/10.1111/nmo.14441
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author López‐Estévez, Sergio
López‐Torrellardona, Josep Manuel
Parera, Marc
Martínez, Vicente
author_facet López‐Estévez, Sergio
López‐Torrellardona, Josep Manuel
Parera, Marc
Martínez, Vicente
author_sort López‐Estévez, Sergio
collection PubMed
description BACKGROUND: Persistent visceral hypersensitivity is a key component of functional and inflammatory gastrointestinal diseases. Current animal models fail to fully reproduce the characteristics of visceral pain in humans, particularly as it relates to persistent hypersensitivity. This work explores the validity of DSS‐induced colitis in rats as a model to mimic chronic intestinal hypersensitivity. METHODS: Exposure to DSS (5% for 7 days) was used to induce colitis in rats. Thereafter, changes in viscerosensitivity (visceromotor responses to colorectal distension—CRD), the presence of somatic referred pain (mechanosensitivity of the hind paws, von Frey test) and the expression (qRT‐PCR) of sensory‐related markers (colon, lumbosacral DRGs, and lumbosacral spinal cord) were assessed at different times during the 35 days period after colitis induction. RESULTS: Following colitis, a sustained increase in visceromotor responses to CRD were observed, indicative of the presence of visceral hypersensitivity. Responses in animals without colitis remained stable over time. In colitic animals, somatic referred hypersensitivity was also detected. DSS‐induced colitis was associated to a differential expression of sensory‐related markers (with both pro‐ and anti‐nociceptive action) in the colon, lumbosacral DRGs and lumbosacral spinal cord; indicating the presence of peripheral and central sensitization. CONCLUSIONS AND INFERENCES: DSS‐induced colitis in rats is associated to the generation of a long‐lasting state of visceral (colonic) hypersensitivity, despite clinical colitis resolution. This model reproduces the changes in intestinal sensitivity characteristics of inflammatory and functional gastrointestinal disorders in humans and can be used in the characterization of new pharmacological treatments against visceral pain.
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spelling pubmed-97877592022-12-28 Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats López‐Estévez, Sergio López‐Torrellardona, Josep Manuel Parera, Marc Martínez, Vicente Neurogastroenterol Motil Original Articles BACKGROUND: Persistent visceral hypersensitivity is a key component of functional and inflammatory gastrointestinal diseases. Current animal models fail to fully reproduce the characteristics of visceral pain in humans, particularly as it relates to persistent hypersensitivity. This work explores the validity of DSS‐induced colitis in rats as a model to mimic chronic intestinal hypersensitivity. METHODS: Exposure to DSS (5% for 7 days) was used to induce colitis in rats. Thereafter, changes in viscerosensitivity (visceromotor responses to colorectal distension—CRD), the presence of somatic referred pain (mechanosensitivity of the hind paws, von Frey test) and the expression (qRT‐PCR) of sensory‐related markers (colon, lumbosacral DRGs, and lumbosacral spinal cord) were assessed at different times during the 35 days period after colitis induction. RESULTS: Following colitis, a sustained increase in visceromotor responses to CRD were observed, indicative of the presence of visceral hypersensitivity. Responses in animals without colitis remained stable over time. In colitic animals, somatic referred hypersensitivity was also detected. DSS‐induced colitis was associated to a differential expression of sensory‐related markers (with both pro‐ and anti‐nociceptive action) in the colon, lumbosacral DRGs and lumbosacral spinal cord; indicating the presence of peripheral and central sensitization. CONCLUSIONS AND INFERENCES: DSS‐induced colitis in rats is associated to the generation of a long‐lasting state of visceral (colonic) hypersensitivity, despite clinical colitis resolution. This model reproduces the changes in intestinal sensitivity characteristics of inflammatory and functional gastrointestinal disorders in humans and can be used in the characterization of new pharmacological treatments against visceral pain. John Wiley and Sons Inc. 2022-08-05 2022-11 /pmc/articles/PMC9787759/ /pubmed/36239298 http://dx.doi.org/10.1111/nmo.14441 Text en © 2022 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
López‐Estévez, Sergio
López‐Torrellardona, Josep Manuel
Parera, Marc
Martínez, Vicente
Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title_full Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title_fullStr Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title_full_unstemmed Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title_short Long‐lasting visceral hypersensitivity in a model of DSS‐induced colitis in rats
title_sort long‐lasting visceral hypersensitivity in a model of dss‐induced colitis in rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787759/
https://www.ncbi.nlm.nih.gov/pubmed/36239298
http://dx.doi.org/10.1111/nmo.14441
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