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Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis

SCOPE: While cocoa flavanol (CF) consumption improves cardiovascular risk biomarkers, molecular mechanisms underlying their protective effects are not understood. OBJECTIVE: To investigate nutri(epi)genomic effects of CF and identify regulatory networks potential mediating vascular health benefits....

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Autores principales: Milenkovic, Dragan, Rodriguez‐Mateos, Ana, Lucosz, Margarete, Istas, Geoffrey, Declerck, Ken, Sansone, Roberto, Deenen, René, Köhrer, Karl, Corral‐Jara, Karla Fabiola, Altschmied, Joachim, Haendeler, Judith, Kelm, Malte, Berghe, Wim Vanden, Heiss, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787825/
https://www.ncbi.nlm.nih.gov/pubmed/35094491
http://dx.doi.org/10.1002/mnfr.202100991
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author Milenkovic, Dragan
Rodriguez‐Mateos, Ana
Lucosz, Margarete
Istas, Geoffrey
Declerck, Ken
Sansone, Roberto
Deenen, René
Köhrer, Karl
Corral‐Jara, Karla Fabiola
Altschmied, Joachim
Haendeler, Judith
Kelm, Malte
Berghe, Wim Vanden
Heiss, Christian
author_facet Milenkovic, Dragan
Rodriguez‐Mateos, Ana
Lucosz, Margarete
Istas, Geoffrey
Declerck, Ken
Sansone, Roberto
Deenen, René
Köhrer, Karl
Corral‐Jara, Karla Fabiola
Altschmied, Joachim
Haendeler, Judith
Kelm, Malte
Berghe, Wim Vanden
Heiss, Christian
author_sort Milenkovic, Dragan
collection PubMed
description SCOPE: While cocoa flavanol (CF) consumption improves cardiovascular risk biomarkers, molecular mechanisms underlying their protective effects are not understood. OBJECTIVE: To investigate nutri(epi)genomic effects of CF and identify regulatory networks potential mediating vascular health benefits. METHODS AND RESULTS: Twenty healthy middle‐aged men consume CF (bi‐daily 450 mg) or control drinks for 1 month. Microarray analysis identifies 2235 differentially expressed genes (DEG) involved in processes regulating immune response, cell adhesion, or cytoskeleton organization. Distinct patterns of DEG correlate with CF‐related changes in endothelial function, arterial stiffness, and blood pressure. DEG profile negatively correlates with expression profiles of cardiovascular disease patients. CF modulated DNA methylation profile of genes implicates in cell adhesion, actin cytoskeleton organization, or cell signaling. In silico docking analyses indicate that CF metabolites have the potential of binding to cell signaling proteins and transcription factors. Incubation of plasma obtained after CF consumption decrease monocyte to endothelial adhesion and dose‐dependently increase nitric oxide‐dependent chemotaxis of circulating angiogenic cells further validating the biological functions of CF metabolites. CONCLUSION: In healthy humans, CF consumption may mediate vascular protective effects by modulating gene expression and DNA methylation towards a cardiovascular protective effect, in agreement with clinical results, by preserving integrity of immunological‐endothelial barrier functions.
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spelling pubmed-97878252022-12-28 Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis Milenkovic, Dragan Rodriguez‐Mateos, Ana Lucosz, Margarete Istas, Geoffrey Declerck, Ken Sansone, Roberto Deenen, René Köhrer, Karl Corral‐Jara, Karla Fabiola Altschmied, Joachim Haendeler, Judith Kelm, Malte Berghe, Wim Vanden Heiss, Christian Mol Nutr Food Res Research Articles SCOPE: While cocoa flavanol (CF) consumption improves cardiovascular risk biomarkers, molecular mechanisms underlying their protective effects are not understood. OBJECTIVE: To investigate nutri(epi)genomic effects of CF and identify regulatory networks potential mediating vascular health benefits. METHODS AND RESULTS: Twenty healthy middle‐aged men consume CF (bi‐daily 450 mg) or control drinks for 1 month. Microarray analysis identifies 2235 differentially expressed genes (DEG) involved in processes regulating immune response, cell adhesion, or cytoskeleton organization. Distinct patterns of DEG correlate with CF‐related changes in endothelial function, arterial stiffness, and blood pressure. DEG profile negatively correlates with expression profiles of cardiovascular disease patients. CF modulated DNA methylation profile of genes implicates in cell adhesion, actin cytoskeleton organization, or cell signaling. In silico docking analyses indicate that CF metabolites have the potential of binding to cell signaling proteins and transcription factors. Incubation of plasma obtained after CF consumption decrease monocyte to endothelial adhesion and dose‐dependently increase nitric oxide‐dependent chemotaxis of circulating angiogenic cells further validating the biological functions of CF metabolites. CONCLUSION: In healthy humans, CF consumption may mediate vascular protective effects by modulating gene expression and DNA methylation towards a cardiovascular protective effect, in agreement with clinical results, by preserving integrity of immunological‐endothelial barrier functions. John Wiley and Sons Inc. 2022-02-16 2022-11 /pmc/articles/PMC9787825/ /pubmed/35094491 http://dx.doi.org/10.1002/mnfr.202100991 Text en © 2022 The Authors. Molecular Nutrition & Food Research published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Milenkovic, Dragan
Rodriguez‐Mateos, Ana
Lucosz, Margarete
Istas, Geoffrey
Declerck, Ken
Sansone, Roberto
Deenen, René
Köhrer, Karl
Corral‐Jara, Karla Fabiola
Altschmied, Joachim
Haendeler, Judith
Kelm, Malte
Berghe, Wim Vanden
Heiss, Christian
Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title_full Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title_fullStr Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title_full_unstemmed Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title_short Flavanol Consumption in Healthy Men Preserves Integrity of Immunological‐Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis
title_sort flavanol consumption in healthy men preserves integrity of immunological‐endothelial barrier cell functions: nutri(epi)genomic analysis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787825/
https://www.ncbi.nlm.nih.gov/pubmed/35094491
http://dx.doi.org/10.1002/mnfr.202100991
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