Cargando…
Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon
SCOPE: Colon metabolomes associated with high‐fat (H) versus energy‐restricted (E) diets in early colorectal cancer (CRC) models have never been directly compared. The objectives of this study are to elucidate metabolites associated with diet, aberrant crypt foci (ACF), and diet:ACF interaction, usi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787839/ https://www.ncbi.nlm.nih.gov/pubmed/35969485 http://dx.doi.org/10.1002/mnfr.202200180 |
_version_ | 1784858609458348032 |
---|---|
author | Chatelaine, Haley A. Ramazani, Cynthia A. Spencer, Kyle Olivo‐Marston, Susan Bailey, Michael T. McElroy, Joseph Hatzakis, Emmanuel Mathé, Ewy A. Kopec, Rachel E. |
author_facet | Chatelaine, Haley A. Ramazani, Cynthia A. Spencer, Kyle Olivo‐Marston, Susan Bailey, Michael T. McElroy, Joseph Hatzakis, Emmanuel Mathé, Ewy A. Kopec, Rachel E. |
author_sort | Chatelaine, Haley A. |
collection | PubMed |
description | SCOPE: Colon metabolomes associated with high‐fat (H) versus energy‐restricted (E) diets in early colorectal cancer (CRC) models have never been directly compared. The objectives of this study are to elucidate metabolites associated with diet, aberrant crypt foci (ACF), and diet:ACF interaction, using a lifetime murine model. METHODS AND RESULTS: Three‐week‐old mice consumed control (C), E, or H initiation diets for 18 weeks. ACF formation is initiated weeks 16–21 with azoxymethane injections, followed by progression diet crossover (to C, E, or H) through week 60. Colon extracts are analyzed using ultra‐high‐performance liquid chromatography‐high resolution mass spectrometry (UHPLC‐HRMS). Metabolites associated with diet, ACF, or diet:ACF are determined using regression models (FDR‐adjusted p‐value <0.05). No metabolites are significantly associated with initiation diets, but concentrations of acylcarnitines and phospholipids are associated with C, E, and H progression diets. Purines, taurine, and phospholipids are associated with ACF presence. No significant associations between metabolites and diet:ACF interaction are observed. CONCLUSIONS: These results suggest that recent, rather than early‐life, diet is more closely associated with the colon metabolome, particularly lipid metabolism. Results from this study also provide candidate biomarkers of early CRC development and provide support for the importance of early diet on influencing pre‐CRC risk. |
format | Online Article Text |
id | pubmed-9787839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97878392022-12-28 Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon Chatelaine, Haley A. Ramazani, Cynthia A. Spencer, Kyle Olivo‐Marston, Susan Bailey, Michael T. McElroy, Joseph Hatzakis, Emmanuel Mathé, Ewy A. Kopec, Rachel E. Mol Nutr Food Res Research Articles SCOPE: Colon metabolomes associated with high‐fat (H) versus energy‐restricted (E) diets in early colorectal cancer (CRC) models have never been directly compared. The objectives of this study are to elucidate metabolites associated with diet, aberrant crypt foci (ACF), and diet:ACF interaction, using a lifetime murine model. METHODS AND RESULTS: Three‐week‐old mice consumed control (C), E, or H initiation diets for 18 weeks. ACF formation is initiated weeks 16–21 with azoxymethane injections, followed by progression diet crossover (to C, E, or H) through week 60. Colon extracts are analyzed using ultra‐high‐performance liquid chromatography‐high resolution mass spectrometry (UHPLC‐HRMS). Metabolites associated with diet, ACF, or diet:ACF are determined using regression models (FDR‐adjusted p‐value <0.05). No metabolites are significantly associated with initiation diets, but concentrations of acylcarnitines and phospholipids are associated with C, E, and H progression diets. Purines, taurine, and phospholipids are associated with ACF presence. No significant associations between metabolites and diet:ACF interaction are observed. CONCLUSIONS: These results suggest that recent, rather than early‐life, diet is more closely associated with the colon metabolome, particularly lipid metabolism. Results from this study also provide candidate biomarkers of early CRC development and provide support for the importance of early diet on influencing pre‐CRC risk. John Wiley and Sons Inc. 2022-10-21 2022-10 /pmc/articles/PMC9787839/ /pubmed/35969485 http://dx.doi.org/10.1002/mnfr.202200180 Text en © 2022 The Authors. Molecular Nutrition & Food Research published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Chatelaine, Haley A. Ramazani, Cynthia A. Spencer, Kyle Olivo‐Marston, Susan Bailey, Michael T. McElroy, Joseph Hatzakis, Emmanuel Mathé, Ewy A. Kopec, Rachel E. Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title | Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title_full | Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title_fullStr | Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title_full_unstemmed | Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title_short | Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon |
title_sort | dietary energy intake and presence of aberrant crypt foci are associated with phospholipid, purine, and taurine metabolite abundances in c57bl/6n mouse colon |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9787839/ https://www.ncbi.nlm.nih.gov/pubmed/35969485 http://dx.doi.org/10.1002/mnfr.202200180 |
work_keys_str_mv | AT chatelainehaleya dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT ramazanicynthiaa dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT spencerkyle dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT olivomarstonsusan dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT baileymichaelt dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT mcelroyjoseph dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT hatzakisemmanuel dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT matheewya dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon AT kopecrachele dietaryenergyintakeandpresenceofaberrantcryptfociareassociatedwithphospholipidpurineandtaurinemetaboliteabundancesinc57bl6nmousecolon |