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Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models

The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a h...

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Autores principales: Stanojkovic, Tatjana P., Filimonova, Marina, Grozdanic, Nadja, Petovic, Slavica, Shitova, Anna, Soldatova, Olga, Filimonov, Alexander, Vladic, Jelena, Shegay, Petr, Kaprin, Andrey, Ivanov, Sergey, Nikitovic, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788264/
https://www.ncbi.nlm.nih.gov/pubmed/36558184
http://dx.doi.org/10.3390/molecules27249048
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author Stanojkovic, Tatjana P.
Filimonova, Marina
Grozdanic, Nadja
Petovic, Slavica
Shitova, Anna
Soldatova, Olga
Filimonov, Alexander
Vladic, Jelena
Shegay, Petr
Kaprin, Andrey
Ivanov, Sergey
Nikitovic, Marina
author_facet Stanojkovic, Tatjana P.
Filimonova, Marina
Grozdanic, Nadja
Petovic, Slavica
Shitova, Anna
Soldatova, Olga
Filimonov, Alexander
Vladic, Jelena
Shegay, Petr
Kaprin, Andrey
Ivanov, Sergey
Nikitovic, Marina
author_sort Stanojkovic, Tatjana P.
collection PubMed
description The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a human cervix adenocarcinoma cell line (HeLa), human colon adenocarcinoma (LS174), human non-small-cell lung carcinoma (A549), and a normal human fetal lung fibroblast cell line (MRC-5) were used. The in vivo antitumor activity was investigated against two transplantable mouse tumors, the Ehrlich carcinoma (EC) and cervical cancer (CC-5). The effect of avarol on cancer cell survival, which was determined by the microculture tetrazolium test, confirmed a significant in vitro potency of avarol against the investigated cell lines, without selectivity towards MRC-5. The highest cytotoxicity was exhibited against HeLa cancer cells (10.22 ± 0.28 μg/mL). Moreover, potent antitumor activity against two tumor models was determined, as the intraperitoneal administration of avarol at a dose of 50 mg/kg resulted in a significant inhibition of tumor growth in mice. After three administrations of avarol, a 29% inhibition of the EC growth was achieved, while in the case of CC-5, a 36% inhibition of the tumor growth was achieved after the second administration of avarol. Therefore, the results indicate that this marine sesquiterpenoid hydroquinone could be a promising bioactive compound in the development of new anticancer medicine.
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spelling pubmed-97882642022-12-24 Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models Stanojkovic, Tatjana P. Filimonova, Marina Grozdanic, Nadja Petovic, Slavica Shitova, Anna Soldatova, Olga Filimonov, Alexander Vladic, Jelena Shegay, Petr Kaprin, Andrey Ivanov, Sergey Nikitovic, Marina Molecules Article The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a human cervix adenocarcinoma cell line (HeLa), human colon adenocarcinoma (LS174), human non-small-cell lung carcinoma (A549), and a normal human fetal lung fibroblast cell line (MRC-5) were used. The in vivo antitumor activity was investigated against two transplantable mouse tumors, the Ehrlich carcinoma (EC) and cervical cancer (CC-5). The effect of avarol on cancer cell survival, which was determined by the microculture tetrazolium test, confirmed a significant in vitro potency of avarol against the investigated cell lines, without selectivity towards MRC-5. The highest cytotoxicity was exhibited against HeLa cancer cells (10.22 ± 0.28 μg/mL). Moreover, potent antitumor activity against two tumor models was determined, as the intraperitoneal administration of avarol at a dose of 50 mg/kg resulted in a significant inhibition of tumor growth in mice. After three administrations of avarol, a 29% inhibition of the EC growth was achieved, while in the case of CC-5, a 36% inhibition of the tumor growth was achieved after the second administration of avarol. Therefore, the results indicate that this marine sesquiterpenoid hydroquinone could be a promising bioactive compound in the development of new anticancer medicine. MDPI 2022-12-19 /pmc/articles/PMC9788264/ /pubmed/36558184 http://dx.doi.org/10.3390/molecules27249048 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stanojkovic, Tatjana P.
Filimonova, Marina
Grozdanic, Nadja
Petovic, Slavica
Shitova, Anna
Soldatova, Olga
Filimonov, Alexander
Vladic, Jelena
Shegay, Petr
Kaprin, Andrey
Ivanov, Sergey
Nikitovic, Marina
Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title_full Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title_fullStr Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title_full_unstemmed Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title_short Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models
title_sort evaluation of in vitro cytotoxic potential of avarol towards human cancer cell lines and in vivo antitumor activity in solid tumor models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788264/
https://www.ncbi.nlm.nih.gov/pubmed/36558184
http://dx.doi.org/10.3390/molecules27249048
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