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Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing
Background and Objectives: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus’s ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficie...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788413/ https://www.ncbi.nlm.nih.gov/pubmed/36557043 http://dx.doi.org/10.3390/medicina58121841 |
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author | Capraru, Ionut Dragos Romanescu, Mirabela Anghel, Flavia Medana Oancea, Cristian Marian, Catalin Sirbu, Ioan Ovidiu Chis, Aimee Rodica Ciordas, Paula Diana |
author_facet | Capraru, Ionut Dragos Romanescu, Mirabela Anghel, Flavia Medana Oancea, Cristian Marian, Catalin Sirbu, Ioan Ovidiu Chis, Aimee Rodica Ciordas, Paula Diana |
author_sort | Capraru, Ionut Dragos |
collection | PubMed |
description | Background and Objectives: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus’s ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. Materials and Methods: In this study, Nanopore and Ion Torrent sequencing technologies were used to detect the main SARS-CoV-2 circulating strains in Timis County, Romania, between February 2021 and May 2022. Results: We identified 22 virus lineages belonging to seven clades: 20A, 20I (Alpha, V1), 21B (Kappa), 21I (Delta), 21J (Delta), 21K (Omicron), and 21L (Omicron). Conclusions: Results obtained with both methods are comparable, and we confirm the utility of Nanopore sequencing in large-scale epidemiological surveillance due to the lower cost and reduced time for library preparation. |
format | Online Article Text |
id | pubmed-9788413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97884132022-12-24 Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing Capraru, Ionut Dragos Romanescu, Mirabela Anghel, Flavia Medana Oancea, Cristian Marian, Catalin Sirbu, Ioan Ovidiu Chis, Aimee Rodica Ciordas, Paula Diana Medicina (Kaunas) Article Background and Objectives: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus’s ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. Materials and Methods: In this study, Nanopore and Ion Torrent sequencing technologies were used to detect the main SARS-CoV-2 circulating strains in Timis County, Romania, between February 2021 and May 2022. Results: We identified 22 virus lineages belonging to seven clades: 20A, 20I (Alpha, V1), 21B (Kappa), 21I (Delta), 21J (Delta), 21K (Omicron), and 21L (Omicron). Conclusions: Results obtained with both methods are comparable, and we confirm the utility of Nanopore sequencing in large-scale epidemiological surveillance due to the lower cost and reduced time for library preparation. MDPI 2022-12-15 /pmc/articles/PMC9788413/ /pubmed/36557043 http://dx.doi.org/10.3390/medicina58121841 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Capraru, Ionut Dragos Romanescu, Mirabela Anghel, Flavia Medana Oancea, Cristian Marian, Catalin Sirbu, Ioan Ovidiu Chis, Aimee Rodica Ciordas, Paula Diana Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title | Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title_full | Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title_fullStr | Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title_full_unstemmed | Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title_short | Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing |
title_sort | identification of genomic variants of sars-cov-2 using nanopore sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788413/ https://www.ncbi.nlm.nih.gov/pubmed/36557043 http://dx.doi.org/10.3390/medicina58121841 |
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