Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation

Viscum album L., popularly known as mistletoe, is well known for its anti-cancer properties, and the pharmaceutical application of hydroalcoholic dry extracts is still limited due to its low solubility in aqueous media, and physicochemical instability. The Pluronic(®) F127 is an amphiphilic polymer,...

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Autores principales: Rocha, Mariana S., Batista, João V. C., Melo, Michelle N. O., de Campos, Vania E. B., Toledo, Anna Lecticia M. M., Oliveira, Adriana P., Picciani, Paulo H. S., Baumgartner, Stephan, Holandino, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788499/
https://www.ncbi.nlm.nih.gov/pubmed/36559269
http://dx.doi.org/10.3390/pharmaceutics14122775
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author Rocha, Mariana S.
Batista, João V. C.
Melo, Michelle N. O.
de Campos, Vania E. B.
Toledo, Anna Lecticia M. M.
Oliveira, Adriana P.
Picciani, Paulo H. S.
Baumgartner, Stephan
Holandino, Carla
author_facet Rocha, Mariana S.
Batista, João V. C.
Melo, Michelle N. O.
de Campos, Vania E. B.
Toledo, Anna Lecticia M. M.
Oliveira, Adriana P.
Picciani, Paulo H. S.
Baumgartner, Stephan
Holandino, Carla
author_sort Rocha, Mariana S.
collection PubMed
description Viscum album L., popularly known as mistletoe, is well known for its anti-cancer properties, and the pharmaceutical application of hydroalcoholic dry extracts is still limited due to its low solubility in aqueous media, and physicochemical instability. The Pluronic(®) F127 is an amphiphilic polymer, which permits the solubilization of lipophilic and hydrophilic compounds. In this investigation, physicochemical features of hydrogel containing V. album dry extract (VADE-loaded-hydrogel) were performed by: dynamic light scattering (DLS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and transmission electron microscopy (TEM). VADE-loaded-hydrogel presented nanometer-size micelles with volume distribution ranging from 10.58 nm to 246.7 nm, and a polydispersity index of 0.441. The sample thermal analyses (TG and DSC) showed similar decomposition curves; however, the thermal events indicated an increase in thermal stability in relation to the presence of the extract. In addition to these interesting pharmaceutical features, IC(50) values of 333.40 µg/mL and >1000 µg/mL were obtained when tumor (SCC-25) and non-tumor (L929) cells were incubated with VADE-loaded-hydrogel, respectively. The optical and ultrastructural cellular analysis confirmed the tumor selectivity since the following alterations were detected only in SCC-25 cells: disorganization of plasmatic membrane; an increase of cytoplasmatic vacuole size; alteration in the cristae mitochondrial shape; and generation of amorphous cellular material. These results emphasize the promising antitumoral potential of VADE-loaded-hydrogel as an herbal drug delivery system via in vitro assays.
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spelling pubmed-97884992022-12-24 Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation Rocha, Mariana S. Batista, João V. C. Melo, Michelle N. O. de Campos, Vania E. B. Toledo, Anna Lecticia M. M. Oliveira, Adriana P. Picciani, Paulo H. S. Baumgartner, Stephan Holandino, Carla Pharmaceutics Article Viscum album L., popularly known as mistletoe, is well known for its anti-cancer properties, and the pharmaceutical application of hydroalcoholic dry extracts is still limited due to its low solubility in aqueous media, and physicochemical instability. The Pluronic(®) F127 is an amphiphilic polymer, which permits the solubilization of lipophilic and hydrophilic compounds. In this investigation, physicochemical features of hydrogel containing V. album dry extract (VADE-loaded-hydrogel) were performed by: dynamic light scattering (DLS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and transmission electron microscopy (TEM). VADE-loaded-hydrogel presented nanometer-size micelles with volume distribution ranging from 10.58 nm to 246.7 nm, and a polydispersity index of 0.441. The sample thermal analyses (TG and DSC) showed similar decomposition curves; however, the thermal events indicated an increase in thermal stability in relation to the presence of the extract. In addition to these interesting pharmaceutical features, IC(50) values of 333.40 µg/mL and >1000 µg/mL were obtained when tumor (SCC-25) and non-tumor (L929) cells were incubated with VADE-loaded-hydrogel, respectively. The optical and ultrastructural cellular analysis confirmed the tumor selectivity since the following alterations were detected only in SCC-25 cells: disorganization of plasmatic membrane; an increase of cytoplasmatic vacuole size; alteration in the cristae mitochondrial shape; and generation of amorphous cellular material. These results emphasize the promising antitumoral potential of VADE-loaded-hydrogel as an herbal drug delivery system via in vitro assays. MDPI 2022-12-12 /pmc/articles/PMC9788499/ /pubmed/36559269 http://dx.doi.org/10.3390/pharmaceutics14122775 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rocha, Mariana S.
Batista, João V. C.
Melo, Michelle N. O.
de Campos, Vania E. B.
Toledo, Anna Lecticia M. M.
Oliveira, Adriana P.
Picciani, Paulo H. S.
Baumgartner, Stephan
Holandino, Carla
Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title_full Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title_fullStr Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title_full_unstemmed Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title_short Pluronic(®) F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation
title_sort pluronic(®) f127 thermoresponsive viscum album hydrogel: physicochemical features and cellular in vitro evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788499/
https://www.ncbi.nlm.nih.gov/pubmed/36559269
http://dx.doi.org/10.3390/pharmaceutics14122775
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