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Specific isotopic labelling and reverse labelling for protein NMR spectroscopy: using metabolic precursors in sample preparation
The study of protein structure, dynamics and function by NMR spectroscopy commonly requires samples that have been enriched (‘labelled') with the stable isotopes (13)C and/or (15)N. The standard approach is to uniformly label a protein with one or both of these nuclei such that all C and/or N s...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Portland Press Ltd.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788560/ https://www.ncbi.nlm.nih.gov/pubmed/36382942 http://dx.doi.org/10.1042/BST20210586 |