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The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat

Long-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from Pinctada martensii meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, Pinctada martensii meat hydrolysates (PME) were prepared by digestive enzymes and...

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Autores principales: Wei, Mengfen, Qiu, Huamai, Zhou, Jie, Yang, Chenghao, Chen, Yifan, You, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788596/
https://www.ncbi.nlm.nih.gov/pubmed/36547917
http://dx.doi.org/10.3390/md20120770
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author Wei, Mengfen
Qiu, Huamai
Zhou, Jie
Yang, Chenghao
Chen, Yifan
You, Lijun
author_facet Wei, Mengfen
Qiu, Huamai
Zhou, Jie
Yang, Chenghao
Chen, Yifan
You, Lijun
author_sort Wei, Mengfen
collection PubMed
description Long-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from Pinctada martensii meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, Pinctada martensii meat hydrolysates (PME) were prepared by digestive enzymes and then separated by ultrafiltration and Sephadex G-25 gel filtration chromatography to obtain a purified fraction (G2). The fraction G2 was identified by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), and peptide sequences were synthesized by solid-phase synthesis. The mechanism of anti-photoaging activities was investigated using a human immortalised epidermal (HaCaT) cell model. Results showed that peptides from Pinctada martensii meat increased UVB-induced cell viability and reduced the contents of interstitial collagenase (MMP-1) and matrix lysing enzyme (MMP-3) in HaCaT cells. Furthermore, the fraction of G2 significantly downregulated the expression of p38, EKR, JNK, MMP-1, and MMP-3 in HaCaT cells. The peptide sequences Phe-His (FH), Ala-Leu (AL), Met-Tyr (MY), Ala-Gly-Phe (AGF), and Ile-Tyr-Pro (IYP) were identified and synthesized. Besides, FH reduced the contents of MMP-1 and MMP-3 in HaCaT cells, combining them effectively in molecular docking analysis. Thus, peptides from Pinctada martensii meat showed anti-photoaging activities and might have the potential to be used as an anti-photoaging agent in functional foods.
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spelling pubmed-97885962022-12-24 The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat Wei, Mengfen Qiu, Huamai Zhou, Jie Yang, Chenghao Chen, Yifan You, Lijun Mar Drugs Article Long-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from Pinctada martensii meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, Pinctada martensii meat hydrolysates (PME) were prepared by digestive enzymes and then separated by ultrafiltration and Sephadex G-25 gel filtration chromatography to obtain a purified fraction (G2). The fraction G2 was identified by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), and peptide sequences were synthesized by solid-phase synthesis. The mechanism of anti-photoaging activities was investigated using a human immortalised epidermal (HaCaT) cell model. Results showed that peptides from Pinctada martensii meat increased UVB-induced cell viability and reduced the contents of interstitial collagenase (MMP-1) and matrix lysing enzyme (MMP-3) in HaCaT cells. Furthermore, the fraction of G2 significantly downregulated the expression of p38, EKR, JNK, MMP-1, and MMP-3 in HaCaT cells. The peptide sequences Phe-His (FH), Ala-Leu (AL), Met-Tyr (MY), Ala-Gly-Phe (AGF), and Ile-Tyr-Pro (IYP) were identified and synthesized. Besides, FH reduced the contents of MMP-1 and MMP-3 in HaCaT cells, combining them effectively in molecular docking analysis. Thus, peptides from Pinctada martensii meat showed anti-photoaging activities and might have the potential to be used as an anti-photoaging agent in functional foods. MDPI 2022-12-08 /pmc/articles/PMC9788596/ /pubmed/36547917 http://dx.doi.org/10.3390/md20120770 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Mengfen
Qiu, Huamai
Zhou, Jie
Yang, Chenghao
Chen, Yifan
You, Lijun
The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title_full The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title_fullStr The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title_full_unstemmed The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title_short The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
title_sort anti-photoaging activity of peptides from pinctada martensii meat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788596/
https://www.ncbi.nlm.nih.gov/pubmed/36547917
http://dx.doi.org/10.3390/md20120770
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