Cargando…

Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)

Menstrual toxic shock syndrome (mTSS) is a rare life-threatening febrile illness that occurs in women using intravaginal menstrual protection. It is caused by toxic shock syndrome toxin 1 (TSST-1) produced by Staphylococcus aureus, triggering a sudden onset of rash and hypotension, subsequently lead...

Descripción completa

Detalles Bibliográficos
Autores principales: Courçon, Marie, Badiou, Cédric, Louwagie, Mathilde, Etievant, Sibyle, Jaquinod, Michel, Lina, Gérard, Brun, Virginie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788610/
https://www.ncbi.nlm.nih.gov/pubmed/36548783
http://dx.doi.org/10.3390/toxins14120886
_version_ 1784858795838537728
author Courçon, Marie
Badiou, Cédric
Louwagie, Mathilde
Etievant, Sibyle
Jaquinod, Michel
Lina, Gérard
Brun, Virginie
author_facet Courçon, Marie
Badiou, Cédric
Louwagie, Mathilde
Etievant, Sibyle
Jaquinod, Michel
Lina, Gérard
Brun, Virginie
author_sort Courçon, Marie
collection PubMed
description Menstrual toxic shock syndrome (mTSS) is a rare life-threatening febrile illness that occurs in women using intravaginal menstrual protection. It is caused by toxic shock syndrome toxin 1 (TSST-1) produced by Staphylococcus aureus, triggering a sudden onset of rash and hypotension, subsequently leading to multiple organ failure. Detecting TSST-1 and S. aureus virulence factors in menstrual fluid could accelerate the diagnosis and improve therapeutic management of mTSS. However, menstrual fluid is a highly complex matrix, making detection of bacterial toxins challenging. Here, we present a mass-spectrometry-based proteomics workflow for the targeted, quantitative analysis of four S. aureus superantigenic toxins in menstrual fluids (TSST-1, SEA, SEC, and SED). This method was applied to characterize toxin levels in menstrual fluids collected from patients with mTSS and healthy women. Toxins were detectable in samples from patients with mTSS and one healthy donor at concentrations ranging from 0 to 0.46 µg/mL for TSST-1, and 0 to 1.07 µg/mL for SEC. SEA and SED were never detected in clinical specimens, even though many S. aureus strains were positive for the corresponding genes. The method presented here could be used to explore toxin production in vivo in users of intravaginal devices to improve the diagnosis, understanding, and prevention of mTSS.
format Online
Article
Text
id pubmed-9788610
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97886102022-12-24 Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS) Courçon, Marie Badiou, Cédric Louwagie, Mathilde Etievant, Sibyle Jaquinod, Michel Lina, Gérard Brun, Virginie Toxins (Basel) Article Menstrual toxic shock syndrome (mTSS) is a rare life-threatening febrile illness that occurs in women using intravaginal menstrual protection. It is caused by toxic shock syndrome toxin 1 (TSST-1) produced by Staphylococcus aureus, triggering a sudden onset of rash and hypotension, subsequently leading to multiple organ failure. Detecting TSST-1 and S. aureus virulence factors in menstrual fluid could accelerate the diagnosis and improve therapeutic management of mTSS. However, menstrual fluid is a highly complex matrix, making detection of bacterial toxins challenging. Here, we present a mass-spectrometry-based proteomics workflow for the targeted, quantitative analysis of four S. aureus superantigenic toxins in menstrual fluids (TSST-1, SEA, SEC, and SED). This method was applied to characterize toxin levels in menstrual fluids collected from patients with mTSS and healthy women. Toxins were detectable in samples from patients with mTSS and one healthy donor at concentrations ranging from 0 to 0.46 µg/mL for TSST-1, and 0 to 1.07 µg/mL for SEC. SEA and SED were never detected in clinical specimens, even though many S. aureus strains were positive for the corresponding genes. The method presented here could be used to explore toxin production in vivo in users of intravaginal devices to improve the diagnosis, understanding, and prevention of mTSS. MDPI 2022-12-19 /pmc/articles/PMC9788610/ /pubmed/36548783 http://dx.doi.org/10.3390/toxins14120886 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Courçon, Marie
Badiou, Cédric
Louwagie, Mathilde
Etievant, Sibyle
Jaquinod, Michel
Lina, Gérard
Brun, Virginie
Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title_full Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title_fullStr Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title_full_unstemmed Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title_short Targeted Proteomics Analysis of Staphylococcal Superantigenic Toxins in Menstrual Fluid from Women with Menstrual Toxic Shock Syndrome (mTSS)
title_sort targeted proteomics analysis of staphylococcal superantigenic toxins in menstrual fluid from women with menstrual toxic shock syndrome (mtss)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9788610/
https://www.ncbi.nlm.nih.gov/pubmed/36548783
http://dx.doi.org/10.3390/toxins14120886
work_keys_str_mv AT courconmarie targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT badioucedric targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT louwagiemathilde targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT etievantsibyle targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT jaquinodmichel targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT linagerard targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss
AT brunvirginie targetedproteomicsanalysisofstaphylococcalsuperantigenictoxinsinmenstrualfluidfromwomenwithmenstrualtoxicshocksyndromemtss