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The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system

Descemet's stripping automated endothelial keratoplasty (DSAEK) is used for treating corneal endothelial dysfunction, and the postoperative visual acuity outcome depends on the thickness of the graft. We created a simple nomogram using factors affecting the cutting thickness during graft prepar...

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Autores principales: Nishisako, Sota, Yamaguchi, Takefumi, Kusano, Yuki, Higa, Kazunari, Aoki, Dai, Sasaki, Chiaki, Shimazaki, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789079/
https://www.ncbi.nlm.nih.gov/pubmed/36564442
http://dx.doi.org/10.1038/s41598-022-26679-w
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author Nishisako, Sota
Yamaguchi, Takefumi
Kusano, Yuki
Higa, Kazunari
Aoki, Dai
Sasaki, Chiaki
Shimazaki, Jun
author_facet Nishisako, Sota
Yamaguchi, Takefumi
Kusano, Yuki
Higa, Kazunari
Aoki, Dai
Sasaki, Chiaki
Shimazaki, Jun
author_sort Nishisako, Sota
collection PubMed
description Descemet's stripping automated endothelial keratoplasty (DSAEK) is used for treating corneal endothelial dysfunction, and the postoperative visual acuity outcome depends on the thickness of the graft. We created a simple nomogram using factors affecting the cutting thickness during graft preparation via a mechanical microkeratome system for DSAEK. This retrospective study was conducted from May 2018 through October 2022 and included donor eyes cut by automatic methods. We measured the graft thickness, cutting accuracy, and assessed ten variables with donor/cornea-related factors potentially affecting the cutting thickness. Subsequently, we created a simple nomogram. We analyzed 81 donor tissues, and the donor median age was 76 years. The mean central graft thickness was 122.2 μm, with 62% of the grafts that could be cut within the target central graft thickness range. Comparatively, donor corneas from those with cardiac diseases were cut deeper (P = 0.007). The developed nomogram provided a 83% probability of estimating the post-cutting graft thickness within 25 µm. Our nomogram, which considers cause of death, enables reproducible production of graft of a desired thickness. A detailed analysis of donor tissues, including the cause of donor death and the characteristics from pressurization to cutting, will enable more precise DSAEK graft preparation.
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spelling pubmed-97890792022-12-25 The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system Nishisako, Sota Yamaguchi, Takefumi Kusano, Yuki Higa, Kazunari Aoki, Dai Sasaki, Chiaki Shimazaki, Jun Sci Rep Article Descemet's stripping automated endothelial keratoplasty (DSAEK) is used for treating corneal endothelial dysfunction, and the postoperative visual acuity outcome depends on the thickness of the graft. We created a simple nomogram using factors affecting the cutting thickness during graft preparation via a mechanical microkeratome system for DSAEK. This retrospective study was conducted from May 2018 through October 2022 and included donor eyes cut by automatic methods. We measured the graft thickness, cutting accuracy, and assessed ten variables with donor/cornea-related factors potentially affecting the cutting thickness. Subsequently, we created a simple nomogram. We analyzed 81 donor tissues, and the donor median age was 76 years. The mean central graft thickness was 122.2 μm, with 62% of the grafts that could be cut within the target central graft thickness range. Comparatively, donor corneas from those with cardiac diseases were cut deeper (P = 0.007). The developed nomogram provided a 83% probability of estimating the post-cutting graft thickness within 25 µm. Our nomogram, which considers cause of death, enables reproducible production of graft of a desired thickness. A detailed analysis of donor tissues, including the cause of donor death and the characteristics from pressurization to cutting, will enable more precise DSAEK graft preparation. Nature Publishing Group UK 2022-12-23 /pmc/articles/PMC9789079/ /pubmed/36564442 http://dx.doi.org/10.1038/s41598-022-26679-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nishisako, Sota
Yamaguchi, Takefumi
Kusano, Yuki
Higa, Kazunari
Aoki, Dai
Sasaki, Chiaki
Shimazaki, Jun
The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title_full The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title_fullStr The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title_full_unstemmed The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title_short The predictability of graft thickness for Descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
title_sort predictability of graft thickness for descemet’s stripping automated endothelial keratoplasty using a mechanical microkeratome system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789079/
https://www.ncbi.nlm.nih.gov/pubmed/36564442
http://dx.doi.org/10.1038/s41598-022-26679-w
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