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Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans

BACKGROUND: Saliva possesses antiviral activity, with submandibular-sublingual (SMSL) saliva having higher antiviral activity than parotid saliva. Various salivary proteins have inactivating effects on influenza A virus (IAV), but the detailed relationship between antiviral proteins and salivary ant...

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Autores principales: Yamamoto, Kenkichi, Yamamoto, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789508/
https://www.ncbi.nlm.nih.gov/pubmed/36566172
http://dx.doi.org/10.1186/s12903-022-02686-1
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author Yamamoto, Kenkichi
Yamamoto, Shinji
author_facet Yamamoto, Kenkichi
Yamamoto, Shinji
author_sort Yamamoto, Kenkichi
collection PubMed
description BACKGROUND: Saliva possesses antiviral activity, with submandibular-sublingual (SMSL) saliva having higher antiviral activity than parotid saliva. Various salivary proteins have inactivating effects on influenza A virus (IAV), but the detailed relationship between antiviral proteins and salivary anti-IAV activities in the parotid and SMSL glands is unknown. Here, to identify salivary proteins with anti-IAV activity, salivary proteins from parotid and SMSL glands were identified, quantified, and compared using liquid chromatography-mass spectrometry. METHODS: Twelve healthy male volunteers participated in the study. Parotid and SMSL saliva was collected by suction and collection devices. We assessed anti-IAV activities, protein concentrations, and protein-bound sialic acid concentrations in parotid and SMSL saliva. RESULTS: SMSL had significantly higher anti-IAV activity than parotid saliva. SMSL also had higher concentrations of glycoproteins, such as mucin 5B and mucin 7, protein-bound sialic acid, cystatins, and lysozyme C, compared with parotid saliva. Salivary mucin 5B and mucin 7 concentrations significantly positively correlated with the salivary protein-bound sialic acid concentration. Salivary anti-IAV activity significantly positively correlated with protein-bound sialic acid, mucin 5B, mucin 7, cystatin-C, -S, and -SN concentrations. CONCLUSION: Salivary mucins, cystatins, and lysozyme C contribute to the high anti-IAV activity of SMSL saliva.
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spelling pubmed-97895082022-12-25 Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans Yamamoto, Kenkichi Yamamoto, Shinji BMC Oral Health Research BACKGROUND: Saliva possesses antiviral activity, with submandibular-sublingual (SMSL) saliva having higher antiviral activity than parotid saliva. Various salivary proteins have inactivating effects on influenza A virus (IAV), but the detailed relationship between antiviral proteins and salivary anti-IAV activities in the parotid and SMSL glands is unknown. Here, to identify salivary proteins with anti-IAV activity, salivary proteins from parotid and SMSL glands were identified, quantified, and compared using liquid chromatography-mass spectrometry. METHODS: Twelve healthy male volunteers participated in the study. Parotid and SMSL saliva was collected by suction and collection devices. We assessed anti-IAV activities, protein concentrations, and protein-bound sialic acid concentrations in parotid and SMSL saliva. RESULTS: SMSL had significantly higher anti-IAV activity than parotid saliva. SMSL also had higher concentrations of glycoproteins, such as mucin 5B and mucin 7, protein-bound sialic acid, cystatins, and lysozyme C, compared with parotid saliva. Salivary mucin 5B and mucin 7 concentrations significantly positively correlated with the salivary protein-bound sialic acid concentration. Salivary anti-IAV activity significantly positively correlated with protein-bound sialic acid, mucin 5B, mucin 7, cystatin-C, -S, and -SN concentrations. CONCLUSION: Salivary mucins, cystatins, and lysozyme C contribute to the high anti-IAV activity of SMSL saliva. BioMed Central 2022-12-24 /pmc/articles/PMC9789508/ /pubmed/36566172 http://dx.doi.org/10.1186/s12903-022-02686-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yamamoto, Kenkichi
Yamamoto, Shinji
Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title_full Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title_fullStr Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title_full_unstemmed Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title_short Comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
title_sort comparison of proteins with anti-influenza virus effects in parotid and submandibular-sublingual saliva in humans
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789508/
https://www.ncbi.nlm.nih.gov/pubmed/36566172
http://dx.doi.org/10.1186/s12903-022-02686-1
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