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Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity

PURPOSE: Vitamin E (VitE) may be classified in “the first line of defense” against the formation of reactive oxygen species. Its inclusion in nanoemulsions (NEs) is a promising alternative to increase its bioavailability. The aim of this study was to compare O/W NEs including VitE based on Almond or...

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Detalles Bibliográficos
Autores principales: Rinaldi, Federica, Hanieh, Patrizia Nadia, Maurizi, Linda, Longhi, Catia, Uccelletti, Daniela, Schifano, Emily, Del Favero, Elena, Cantù, Laura, Ricci, Caterina, Ammendolia, Maria Grazia, Paolino, Donatella, Froiio, Francesca, Marianecci, Carlotta, Carafa, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789705/
https://www.ncbi.nlm.nih.gov/pubmed/36573206
http://dx.doi.org/10.2147/IJN.S376750
Descripción
Sumario:PURPOSE: Vitamin E (VitE) may be classified in “the first line of defense” against the formation of reactive oxygen species. Its inclusion in nanoemulsions (NEs) is a promising alternative to increase its bioavailability. The aim of this study was to compare O/W NEs including VitE based on Almond or Neem oil, showing themselves antioxidant properties. The potential synergy of the antioxidant activities of oils and vitamin E, co-formulated in NEs, was explored. PATIENTS AND METHODS: NEs have been prepared by sonication and deeply characterized evaluating size, ζ-potential, morphology (TEM and SAXS analyses), oil nanodroplet feature, and stability. Antioxidant activity has been evaluated in vitro, in non-tumorigenic HaCaT keratinocytes, and in vivo through fluorescence analysis of C. elegans transgenic strain. Moreover, on healthy human volunteers, skin tolerability and anti-inflammatory activity were evaluated by measuring the reduction of the skin erythema induced by the application of a skin chemical irritant (methyl-nicotinate). RESULTS: Results confirm that Vitamin E can be formulated in highly stable NEs showing good antioxidant activity on keratinocyte and on C. elegans. Interestingly, only Neem oil NEs showed some anti-inflammatory activity on healthy volunteers. CONCLUSION: From the obtained results, Neem over Almond oil is a more appropriate candidate for further studies on this application.