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Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity

PURPOSE: Vitamin E (VitE) may be classified in “the first line of defense” against the formation of reactive oxygen species. Its inclusion in nanoemulsions (NEs) is a promising alternative to increase its bioavailability. The aim of this study was to compare O/W NEs including VitE based on Almond or...

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Autores principales: Rinaldi, Federica, Hanieh, Patrizia Nadia, Maurizi, Linda, Longhi, Catia, Uccelletti, Daniela, Schifano, Emily, Del Favero, Elena, Cantù, Laura, Ricci, Caterina, Ammendolia, Maria Grazia, Paolino, Donatella, Froiio, Francesca, Marianecci, Carlotta, Carafa, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789705/
https://www.ncbi.nlm.nih.gov/pubmed/36573206
http://dx.doi.org/10.2147/IJN.S376750
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author Rinaldi, Federica
Hanieh, Patrizia Nadia
Maurizi, Linda
Longhi, Catia
Uccelletti, Daniela
Schifano, Emily
Del Favero, Elena
Cantù, Laura
Ricci, Caterina
Ammendolia, Maria Grazia
Paolino, Donatella
Froiio, Francesca
Marianecci, Carlotta
Carafa, Maria
author_facet Rinaldi, Federica
Hanieh, Patrizia Nadia
Maurizi, Linda
Longhi, Catia
Uccelletti, Daniela
Schifano, Emily
Del Favero, Elena
Cantù, Laura
Ricci, Caterina
Ammendolia, Maria Grazia
Paolino, Donatella
Froiio, Francesca
Marianecci, Carlotta
Carafa, Maria
author_sort Rinaldi, Federica
collection PubMed
description PURPOSE: Vitamin E (VitE) may be classified in “the first line of defense” against the formation of reactive oxygen species. Its inclusion in nanoemulsions (NEs) is a promising alternative to increase its bioavailability. The aim of this study was to compare O/W NEs including VitE based on Almond or Neem oil, showing themselves antioxidant properties. The potential synergy of the antioxidant activities of oils and vitamin E, co-formulated in NEs, was explored. PATIENTS AND METHODS: NEs have been prepared by sonication and deeply characterized evaluating size, ζ-potential, morphology (TEM and SAXS analyses), oil nanodroplet feature, and stability. Antioxidant activity has been evaluated in vitro, in non-tumorigenic HaCaT keratinocytes, and in vivo through fluorescence analysis of C. elegans transgenic strain. Moreover, on healthy human volunteers, skin tolerability and anti-inflammatory activity were evaluated by measuring the reduction of the skin erythema induced by the application of a skin chemical irritant (methyl-nicotinate). RESULTS: Results confirm that Vitamin E can be formulated in highly stable NEs showing good antioxidant activity on keratinocyte and on C. elegans. Interestingly, only Neem oil NEs showed some anti-inflammatory activity on healthy volunteers. CONCLUSION: From the obtained results, Neem over Almond oil is a more appropriate candidate for further studies on this application.
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spelling pubmed-97897052022-12-25 Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity Rinaldi, Federica Hanieh, Patrizia Nadia Maurizi, Linda Longhi, Catia Uccelletti, Daniela Schifano, Emily Del Favero, Elena Cantù, Laura Ricci, Caterina Ammendolia, Maria Grazia Paolino, Donatella Froiio, Francesca Marianecci, Carlotta Carafa, Maria Int J Nanomedicine Original Research PURPOSE: Vitamin E (VitE) may be classified in “the first line of defense” against the formation of reactive oxygen species. Its inclusion in nanoemulsions (NEs) is a promising alternative to increase its bioavailability. The aim of this study was to compare O/W NEs including VitE based on Almond or Neem oil, showing themselves antioxidant properties. The potential synergy of the antioxidant activities of oils and vitamin E, co-formulated in NEs, was explored. PATIENTS AND METHODS: NEs have been prepared by sonication and deeply characterized evaluating size, ζ-potential, morphology (TEM and SAXS analyses), oil nanodroplet feature, and stability. Antioxidant activity has been evaluated in vitro, in non-tumorigenic HaCaT keratinocytes, and in vivo through fluorescence analysis of C. elegans transgenic strain. Moreover, on healthy human volunteers, skin tolerability and anti-inflammatory activity were evaluated by measuring the reduction of the skin erythema induced by the application of a skin chemical irritant (methyl-nicotinate). RESULTS: Results confirm that Vitamin E can be formulated in highly stable NEs showing good antioxidant activity on keratinocyte and on C. elegans. Interestingly, only Neem oil NEs showed some anti-inflammatory activity on healthy volunteers. CONCLUSION: From the obtained results, Neem over Almond oil is a more appropriate candidate for further studies on this application. Dove 2022-12-20 /pmc/articles/PMC9789705/ /pubmed/36573206 http://dx.doi.org/10.2147/IJN.S376750 Text en © 2022 Rinaldi et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Rinaldi, Federica
Hanieh, Patrizia Nadia
Maurizi, Linda
Longhi, Catia
Uccelletti, Daniela
Schifano, Emily
Del Favero, Elena
Cantù, Laura
Ricci, Caterina
Ammendolia, Maria Grazia
Paolino, Donatella
Froiio, Francesca
Marianecci, Carlotta
Carafa, Maria
Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title_full Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title_fullStr Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title_full_unstemmed Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title_short Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity
title_sort neem oil or almond oil nanoemulsions for vitamin e delivery: from structural evaluation to in vivo assessment of antioxidant and anti-inflammatory activity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789705/
https://www.ncbi.nlm.nih.gov/pubmed/36573206
http://dx.doi.org/10.2147/IJN.S376750
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