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Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats
BACKGROUND: Dietary fat composition is a potential major factor affecting energy metabolism. Crocodile oil (CO) is rich in mono- and poly-unsaturated fatty acids exhibiting anti-inflammatory and healing properties. AIM: This study investigated different levels of CO consumption on alterations and ex...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789755/ https://www.ncbi.nlm.nih.gov/pubmed/36589394 http://dx.doi.org/10.5455/OVJ.2022.v12.i5.15 |
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author | Parunyakul, Kongphop Srisuksai, Krittika Santativongchai, Pitchaya Charoenlappanit, Sawanya Phaonakrop, Narumon Roytrakul, Sittiruk Tulayakul, Phitsanu Fungfuang, Wirasak |
author_facet | Parunyakul, Kongphop Srisuksai, Krittika Santativongchai, Pitchaya Charoenlappanit, Sawanya Phaonakrop, Narumon Roytrakul, Sittiruk Tulayakul, Phitsanu Fungfuang, Wirasak |
author_sort | Parunyakul, Kongphop |
collection | PubMed |
description | BACKGROUND: Dietary fat composition is a potential major factor affecting energy metabolism. Crocodile oil (CO) is rich in mono- and poly-unsaturated fatty acids exhibiting anti-inflammatory and healing properties. AIM: This study investigated different levels of CO consumption on alterations and expression of proteins involved in energy metabolism in rats. METHODS: Twenty-one male Sprague-Dawley rats were divided into three groups and administered sterile water (N) or different doses of CO [1% or 3% (v/w) CO] orally once daily for 8 weeks. Body weight gain, food intake, energy intake, blood lipid profiles, and serum energy-related metabolites were determined. The serum proteome was analyzed using shotgun proteomics, and the functions of several candidate proteins were classified using PANTHER software. RESULTS: There were no significant differences in body weight or energy intake were observed between groups. However, both CO-treated groups showed significantly decreased serum triglyceride (TG) levels (p < 0.05). Moreover, post-treatment serum TG levels in the 1%CO group were significantly lower than pre-treatment compared with other groups. The serum oxaloacetate level was also significantly higher in both CO groups than in the N group. The proteomic analysis classified 4,525 serum proteins and revealed more unique proteins involved in cellular metabolic activity in both CO-treated groups than in the N group. Self-organizing tree algorithm clustering of 295 shared differentially expressed proteins in both CO-treatment groups showed that upregulated hyper-expressed protein clusters in both CO groups were associated with catalytic activity and molecular activity on the same levels. CONCLUSION: CO simultaneously enhances energy metabolism and improves lipid profiles. |
format | Online Article Text |
id | pubmed-9789755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Faculty of Veterinary Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-97897552022-12-30 Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats Parunyakul, Kongphop Srisuksai, Krittika Santativongchai, Pitchaya Charoenlappanit, Sawanya Phaonakrop, Narumon Roytrakul, Sittiruk Tulayakul, Phitsanu Fungfuang, Wirasak Open Vet J Original Research BACKGROUND: Dietary fat composition is a potential major factor affecting energy metabolism. Crocodile oil (CO) is rich in mono- and poly-unsaturated fatty acids exhibiting anti-inflammatory and healing properties. AIM: This study investigated different levels of CO consumption on alterations and expression of proteins involved in energy metabolism in rats. METHODS: Twenty-one male Sprague-Dawley rats were divided into three groups and administered sterile water (N) or different doses of CO [1% or 3% (v/w) CO] orally once daily for 8 weeks. Body weight gain, food intake, energy intake, blood lipid profiles, and serum energy-related metabolites were determined. The serum proteome was analyzed using shotgun proteomics, and the functions of several candidate proteins were classified using PANTHER software. RESULTS: There were no significant differences in body weight or energy intake were observed between groups. However, both CO-treated groups showed significantly decreased serum triglyceride (TG) levels (p < 0.05). Moreover, post-treatment serum TG levels in the 1%CO group were significantly lower than pre-treatment compared with other groups. The serum oxaloacetate level was also significantly higher in both CO groups than in the N group. The proteomic analysis classified 4,525 serum proteins and revealed more unique proteins involved in cellular metabolic activity in both CO-treated groups than in the N group. Self-organizing tree algorithm clustering of 295 shared differentially expressed proteins in both CO-treatment groups showed that upregulated hyper-expressed protein clusters in both CO groups were associated with catalytic activity and molecular activity on the same levels. CONCLUSION: CO simultaneously enhances energy metabolism and improves lipid profiles. Faculty of Veterinary Medicine 2022 2022-09-18 /pmc/articles/PMC9789755/ /pubmed/36589394 http://dx.doi.org/10.5455/OVJ.2022.v12.i5.15 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Parunyakul, Kongphop Srisuksai, Krittika Santativongchai, Pitchaya Charoenlappanit, Sawanya Phaonakrop, Narumon Roytrakul, Sittiruk Tulayakul, Phitsanu Fungfuang, Wirasak Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title | Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title_full | Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title_fullStr | Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title_full_unstemmed | Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title_short | Serum proteomic analysis reveals the differential dose effects of crocodile oil from Crocodylus siamensis on energy metabolism in rats |
title_sort | serum proteomic analysis reveals the differential dose effects of crocodile oil from crocodylus siamensis on energy metabolism in rats |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789755/ https://www.ncbi.nlm.nih.gov/pubmed/36589394 http://dx.doi.org/10.5455/OVJ.2022.v12.i5.15 |
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