A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.

Ssd1p is an RNA binding protein in Saccharomyces cerevisiae that plays an important role in cell division, cell fate decisions, stress response and virulence. Lab strain W303 encodes the terminal truncation ssd1-2, which is typically interpreted to be a loss of function allele. We have shown that ss...

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Detalles Bibliográficos
Autores principales: Breeden, Linda, Miles, Shawna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790081/
https://www.ncbi.nlm.nih.gov/pubmed/36575737
http://dx.doi.org/10.17912/micropub.biology.000671
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author Breeden, Linda
Miles, Shawna
author_facet Breeden, Linda
Miles, Shawna
author_sort Breeden, Linda
collection PubMed
description Ssd1p is an RNA binding protein in Saccharomyces cerevisiae that plays an important role in cell division, cell fate decisions, stress response and virulence. Lab strain W303 encodes the terminal truncation ssd1-2, which is typically interpreted to be a loss of function allele. We have shown that ssd1-2 is toxic to mpt5-Δ mutants and to diploids entering stationary phase and quiescence. The ssd1-Δ null shows no toxicity, indicating that ssd1-2 is disrupting an essential function that does not solely require Ssd1p. ssd1-2 cells are also more sensitive to stress than ssd1-Δ . These phenotypes are recessive to SSD1-1 . In contrast, ssd1-2 plays a dominant role in promoting sporulation.
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spelling pubmed-97900812022-12-26 A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation. Breeden, Linda Miles, Shawna MicroPubl Biol New Finding Ssd1p is an RNA binding protein in Saccharomyces cerevisiae that plays an important role in cell division, cell fate decisions, stress response and virulence. Lab strain W303 encodes the terminal truncation ssd1-2, which is typically interpreted to be a loss of function allele. We have shown that ssd1-2 is toxic to mpt5-Δ mutants and to diploids entering stationary phase and quiescence. The ssd1-Δ null shows no toxicity, indicating that ssd1-2 is disrupting an essential function that does not solely require Ssd1p. ssd1-2 cells are also more sensitive to stress than ssd1-Δ . These phenotypes are recessive to SSD1-1 . In contrast, ssd1-2 plays a dominant role in promoting sporulation. Caltech Library 2022-12-09 /pmc/articles/PMC9790081/ /pubmed/36575737 http://dx.doi.org/10.17912/micropub.biology.000671 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Breeden, Linda
Miles, Shawna
A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title_full A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title_fullStr A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title_full_unstemmed A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title_short A common SSD1 truncation is toxic to cells entering quiescence and promotes sporulation.
title_sort common ssd1 truncation is toxic to cells entering quiescence and promotes sporulation.
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790081/
https://www.ncbi.nlm.nih.gov/pubmed/36575737
http://dx.doi.org/10.17912/micropub.biology.000671
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