Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans

FOXO transcription factors regulate development, longevity, and stress-resistance across species. The C. elegans FOXO ortholog, daf-16, has three major isoforms with distinct promoters and N-termini. Different combinations of isoforms regulate different processes. Adverse environments can induce dau...

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Autores principales: Cuko, Liberta, Cale, Allison R, Rambo, Loni, Knoblock, Macy L, Karp, Xantha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790082/
https://www.ncbi.nlm.nih.gov/pubmed/36575736
http://dx.doi.org/10.17912/micropub.biology.000706
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author Cuko, Liberta
Cale, Allison R
Rambo, Loni
Knoblock, Macy L
Karp, Xantha
author_facet Cuko, Liberta
Cale, Allison R
Rambo, Loni
Knoblock, Macy L
Karp, Xantha
author_sort Cuko, Liberta
collection PubMed
description FOXO transcription factors regulate development, longevity, and stress-resistance across species. The C. elegans FOXO ortholog, daf-16, has three major isoforms with distinct promoters and N-termini. Different combinations of isoforms regulate different processes. Adverse environments can induce dauer diapause after the second larval molt. During dauer, daf-16 blocks specification of vulval precursor cells, including EGFR/Ras-mediated 1˚ fate specification and LIN-12/Notch-mediated 2˚ fate specification. Using isoform-specific mutants, we find that daf-16a and daf-16f are functionally redundant for the block to the expression of 1˚ fate markers. In contrast, all three isoforms contribute to blocking the expression of 2˚ fate markers.
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spelling pubmed-97900822022-12-26 Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans Cuko, Liberta Cale, Allison R Rambo, Loni Knoblock, Macy L Karp, Xantha MicroPubl Biol New Finding FOXO transcription factors regulate development, longevity, and stress-resistance across species. The C. elegans FOXO ortholog, daf-16, has three major isoforms with distinct promoters and N-termini. Different combinations of isoforms regulate different processes. Adverse environments can induce dauer diapause after the second larval molt. During dauer, daf-16 blocks specification of vulval precursor cells, including EGFR/Ras-mediated 1˚ fate specification and LIN-12/Notch-mediated 2˚ fate specification. Using isoform-specific mutants, we find that daf-16a and daf-16f are functionally redundant for the block to the expression of 1˚ fate markers. In contrast, all three isoforms contribute to blocking the expression of 2˚ fate markers. Caltech Library 2022-12-09 /pmc/articles/PMC9790082/ /pubmed/36575736 http://dx.doi.org/10.17912/micropub.biology.000706 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Cuko, Liberta
Cale, Allison R
Rambo, Loni
Knoblock, Macy L
Karp, Xantha
Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title_full Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title_fullStr Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title_full_unstemmed Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title_short Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans
title_sort distinct daf-16 isoforms regulate specification of vulval precursor cells in caenorhabditis elegans
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790082/
https://www.ncbi.nlm.nih.gov/pubmed/36575736
http://dx.doi.org/10.17912/micropub.biology.000706
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