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Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats

INTRODUCTION: Cell therapy is the most advanced treatment of peripheral nerve injury. This study aimed to determine the effects of transplantation of hair follicle stem cells on the regeneration of the sciatic nerve injury in rats. METHODS: The bulge region of the rat whisker were isolated and cultu...

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Autores principales: Hejazian, Leila Beigom, Akbarnejad, Zeinab, Moghani Ghoroghi, Fatemeh, Esmaeilzade, Banafshe, Chaibakhsh, Samira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790101/
https://www.ncbi.nlm.nih.gov/pubmed/36589026
http://dx.doi.org/10.32598/bcn.2021.2240.1
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author Hejazian, Leila Beigom
Akbarnejad, Zeinab
Moghani Ghoroghi, Fatemeh
Esmaeilzade, Banafshe
Chaibakhsh, Samira
author_facet Hejazian, Leila Beigom
Akbarnejad, Zeinab
Moghani Ghoroghi, Fatemeh
Esmaeilzade, Banafshe
Chaibakhsh, Samira
author_sort Hejazian, Leila Beigom
collection PubMed
description INTRODUCTION: Cell therapy is the most advanced treatment of peripheral nerve injury. This study aimed to determine the effects of transplantation of hair follicle stem cells on the regeneration of the sciatic nerve injury in rats. METHODS: The bulge region of the rat whisker were isolated and cultured. Morphological and biological features of the cultured bulge cells were observed by light microscopy and immunocytochemistry methods. Percentages of CD34, K15, and nestin cell markers expression were demonstrated by flow cytometry. Rats were randomly divided into 3 groups of injury, epineurium, and epineurium with cells in which rat Hair Follicular Stem Cells (rHFSCs) were injected into the site of the nerve cut. HFSCs were labeled with Bromodeoxyuridine (BrdU), and double-labeling immunofluorescence was performed to study the survival and differentiation of the grafted cells. After 8 weeks, electrophysiological, histological, and immunocytochemical analysis assessments were performed. RESULTS: Rat hair follicle stem cells are suitable for cell culture, proliferation, and differentiation. The results suggest that transplantation of rat hair follicle stem cells can regenerate sciatic nerve injury; moreover, electrophysiology and histology examinations show that sciatic nerve repair was more effective in the epineurium with cell group than in the other experimental group (P<0.05). CONCLUSION: The achieved results propose that hair follicle stem cells improve axonal growth and functional recovery after peripheral nerve injury. HIGHLIGHTS: This study showed that rat hair follicle stem cells are suitable for cell culture, proliferation and differentiation. The results suggested that transplantation of rat hair follicle stem cells had the potential capability of regenerating sciatic nerve injury. Evidence of electrophysiology and histology showed Concomitant use of epineurium with hair follicle stem cell was more effective repairment. PLAIN LANGUAGE SUMMARY: Although repairing damaged peripheral nerves has always been a medical challenge, but peripheral nerve injury has been successfully repaired using various procedures such as nerve auto-graft or stem cell therapy. The functional reconstruction is the most important after therapy because of that primary nerve repair or use of nerve autograft, are still accepted as golden standard methods for treatment. Considerable recent interest has been focused on adult stem cells for both research and clinical applications. A highly promising source of relatively abundant and accessible, active, multipotent adult stem cells are obtained from hair follicles. In research the hair follicle stem cells implanted into the gap region of a severed sciatic nerve injury greatly enhanced the rate of nerve regeneration and the restoration of nerve function. Time is one of the several aspects require specific attention in the clinical treatment of peripheral nerve injury. Because delay of nerve injury treatment may cause neurobiological alterations in neurons and Schwann cells, impairing nerve functional recovery and affect neuron survival. In this study, concluded that stem cell injection 2 weeks after injury in the damaged nerve epineurium repairs nerve fibers, while electrophysiology of the leg muscles showed that muscle function was significantly improved. It indicates the repair of muscular innervation and nerve repair. The results pave the way for further research on this topic.
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spelling pubmed-97901012022-12-29 Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats Hejazian, Leila Beigom Akbarnejad, Zeinab Moghani Ghoroghi, Fatemeh Esmaeilzade, Banafshe Chaibakhsh, Samira Basic Clin Neurosci Research Paper INTRODUCTION: Cell therapy is the most advanced treatment of peripheral nerve injury. This study aimed to determine the effects of transplantation of hair follicle stem cells on the regeneration of the sciatic nerve injury in rats. METHODS: The bulge region of the rat whisker were isolated and cultured. Morphological and biological features of the cultured bulge cells were observed by light microscopy and immunocytochemistry methods. Percentages of CD34, K15, and nestin cell markers expression were demonstrated by flow cytometry. Rats were randomly divided into 3 groups of injury, epineurium, and epineurium with cells in which rat Hair Follicular Stem Cells (rHFSCs) were injected into the site of the nerve cut. HFSCs were labeled with Bromodeoxyuridine (BrdU), and double-labeling immunofluorescence was performed to study the survival and differentiation of the grafted cells. After 8 weeks, electrophysiological, histological, and immunocytochemical analysis assessments were performed. RESULTS: Rat hair follicle stem cells are suitable for cell culture, proliferation, and differentiation. The results suggest that transplantation of rat hair follicle stem cells can regenerate sciatic nerve injury; moreover, electrophysiology and histology examinations show that sciatic nerve repair was more effective in the epineurium with cell group than in the other experimental group (P<0.05). CONCLUSION: The achieved results propose that hair follicle stem cells improve axonal growth and functional recovery after peripheral nerve injury. HIGHLIGHTS: This study showed that rat hair follicle stem cells are suitable for cell culture, proliferation and differentiation. The results suggested that transplantation of rat hair follicle stem cells had the potential capability of regenerating sciatic nerve injury. Evidence of electrophysiology and histology showed Concomitant use of epineurium with hair follicle stem cell was more effective repairment. PLAIN LANGUAGE SUMMARY: Although repairing damaged peripheral nerves has always been a medical challenge, but peripheral nerve injury has been successfully repaired using various procedures such as nerve auto-graft or stem cell therapy. The functional reconstruction is the most important after therapy because of that primary nerve repair or use of nerve autograft, are still accepted as golden standard methods for treatment. Considerable recent interest has been focused on adult stem cells for both research and clinical applications. A highly promising source of relatively abundant and accessible, active, multipotent adult stem cells are obtained from hair follicles. In research the hair follicle stem cells implanted into the gap region of a severed sciatic nerve injury greatly enhanced the rate of nerve regeneration and the restoration of nerve function. Time is one of the several aspects require specific attention in the clinical treatment of peripheral nerve injury. Because delay of nerve injury treatment may cause neurobiological alterations in neurons and Schwann cells, impairing nerve functional recovery and affect neuron survival. In this study, concluded that stem cell injection 2 weeks after injury in the damaged nerve epineurium repairs nerve fibers, while electrophysiology of the leg muscles showed that muscle function was significantly improved. It indicates the repair of muscular innervation and nerve repair. The results pave the way for further research on this topic. Iranian Neuroscience Society 2022 2022-01-01 /pmc/articles/PMC9790101/ /pubmed/36589026 http://dx.doi.org/10.32598/bcn.2021.2240.1 Text en Copyright© 2022 Iranian Neuroscience Society https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research Paper
Hejazian, Leila Beigom
Akbarnejad, Zeinab
Moghani Ghoroghi, Fatemeh
Esmaeilzade, Banafshe
Chaibakhsh, Samira
Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title_full Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title_fullStr Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title_full_unstemmed Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title_short Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats
title_sort augmenting peripheral nerve regeneration using hair follicle stem cells in rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790101/
https://www.ncbi.nlm.nih.gov/pubmed/36589026
http://dx.doi.org/10.32598/bcn.2021.2240.1
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