Cargando…
Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances
OBJECTIVES: The aim of this study was to compare the intra‐oral bacterial profile of normal‐weight and obese adolescents prior to orthodontic treatment with fixed appliances. MATERIALS AND METHODS: Nineteen adolescent patients were recruited into two groups based upon body mass index (BMI) and class...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790364/ https://www.ncbi.nlm.nih.gov/pubmed/35298866 http://dx.doi.org/10.1111/ocr.12571 |
_version_ | 1784859159841210368 |
---|---|
author | Sharara, Shima H. Cleaver, Leanne M. Saloom, Hayder Carpenter, Guy H. Cobourne, Martyn T. |
author_facet | Sharara, Shima H. Cleaver, Leanne M. Saloom, Hayder Carpenter, Guy H. Cobourne, Martyn T. |
author_sort | Sharara, Shima H. |
collection | PubMed |
description | OBJECTIVES: The aim of this study was to compare the intra‐oral bacterial profile of normal‐weight and obese adolescents prior to orthodontic treatment with fixed appliances. MATERIALS AND METHODS: Nineteen adolescent patients were recruited into two groups based upon body mass index (BMI) and classified as normal‐weight or obese. Unstimulated whole mouth saliva was obtained for 5 minutes. Bacterial DNA extraction was performed from saliva, and 16S rRNA gene sequencing of the V1‐2 variable regions was undertaken followed by analysis using the mothur pipeline. RESULTS: Saliva from a total of 19 adolescent patients with mean (SD) age 15.6 (1.8) years were divided into 10 normal‐weight with mean BMI of 19.4 (2.2) kg/m(2) and 9 obese with mean BMI of 30.2 (3.5) kg/m(2). A total of 156 783 sequences were obtained from the 19 samples with no significant differences in richness or diversity between sample groups by obesity status or gender (AMOVA). The bacterial community in both groups was dominated by bacterial genera characteristic of the human mouth, which included Streptococcus, Porphyromonas, Veillonella, Gemella, Prevotella, Fusobacterium and Rothia. CONCLUSION: There were no differences in alpha or beta diversity of oral bacterial communities between normal‐weight and obese orthodontic patients. Obese adolescents attending for orthodontic treatment had a similar microflora to their normal‐weight counterparts. |
format | Online Article Text |
id | pubmed-9790364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97903642022-12-28 Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances Sharara, Shima H. Cleaver, Leanne M. Saloom, Hayder Carpenter, Guy H. Cobourne, Martyn T. Orthod Craniofac Res Research Articles OBJECTIVES: The aim of this study was to compare the intra‐oral bacterial profile of normal‐weight and obese adolescents prior to orthodontic treatment with fixed appliances. MATERIALS AND METHODS: Nineteen adolescent patients were recruited into two groups based upon body mass index (BMI) and classified as normal‐weight or obese. Unstimulated whole mouth saliva was obtained for 5 minutes. Bacterial DNA extraction was performed from saliva, and 16S rRNA gene sequencing of the V1‐2 variable regions was undertaken followed by analysis using the mothur pipeline. RESULTS: Saliva from a total of 19 adolescent patients with mean (SD) age 15.6 (1.8) years were divided into 10 normal‐weight with mean BMI of 19.4 (2.2) kg/m(2) and 9 obese with mean BMI of 30.2 (3.5) kg/m(2). A total of 156 783 sequences were obtained from the 19 samples with no significant differences in richness or diversity between sample groups by obesity status or gender (AMOVA). The bacterial community in both groups was dominated by bacterial genera characteristic of the human mouth, which included Streptococcus, Porphyromonas, Veillonella, Gemella, Prevotella, Fusobacterium and Rothia. CONCLUSION: There were no differences in alpha or beta diversity of oral bacterial communities between normal‐weight and obese orthodontic patients. Obese adolescents attending for orthodontic treatment had a similar microflora to their normal‐weight counterparts. John Wiley and Sons Inc. 2022-05-01 2022-11 /pmc/articles/PMC9790364/ /pubmed/35298866 http://dx.doi.org/10.1111/ocr.12571 Text en © 2022 The Authors. Orthodontics & Craniofacial Research published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Sharara, Shima H. Cleaver, Leanne M. Saloom, Hayder Carpenter, Guy H. Cobourne, Martyn T. Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title | Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title_full | Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title_fullStr | Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title_full_unstemmed | Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title_short | Salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
title_sort | salivary bacterial community profile in normal‐weight and obese adolescent patients prior to orthodontic treatment with fixed appliances |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790364/ https://www.ncbi.nlm.nih.gov/pubmed/35298866 http://dx.doi.org/10.1111/ocr.12571 |
work_keys_str_mv | AT shararashimah salivarybacterialcommunityprofileinnormalweightandobeseadolescentpatientspriortoorthodontictreatmentwithfixedappliances AT cleaverleannem salivarybacterialcommunityprofileinnormalweightandobeseadolescentpatientspriortoorthodontictreatmentwithfixedappliances AT saloomhayder salivarybacterialcommunityprofileinnormalweightandobeseadolescentpatientspriortoorthodontictreatmentwithfixedappliances AT carpenterguyh salivarybacterialcommunityprofileinnormalweightandobeseadolescentpatientspriortoorthodontictreatmentwithfixedappliances AT cobournemartynt salivarybacterialcommunityprofileinnormalweightandobeseadolescentpatientspriortoorthodontictreatmentwithfixedappliances |