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Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China

The area around Poyang Lake is the main aquaculture area in Jiangxi Province, China, and an important base for the supply of freshwater aquatic products. Aquaculture in the Poyang Lake area is severely threatened by diseases caused by bacterial pathogens, and Aeromonas veronii has been the main path...

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Autores principales: Xu, Xiandong, Fu, Huiyun, Wan, Guoyuan, Huang, Jiangfeng, Zhou, Zhiyong, Rao, Yi, Liu, Lihui, Wen, Chungen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791064/
https://www.ncbi.nlm.nih.gov/pubmed/36578578
http://dx.doi.org/10.3389/fmicb.2022.1042007
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author Xu, Xiandong
Fu, Huiyun
Wan, Guoyuan
Huang, Jiangfeng
Zhou, Zhiyong
Rao, Yi
Liu, Lihui
Wen, Chungen
author_facet Xu, Xiandong
Fu, Huiyun
Wan, Guoyuan
Huang, Jiangfeng
Zhou, Zhiyong
Rao, Yi
Liu, Lihui
Wen, Chungen
author_sort Xu, Xiandong
collection PubMed
description The area around Poyang Lake is the main aquaculture area in Jiangxi Province, China, and an important base for the supply of freshwater aquatic products. Aquaculture in the Poyang Lake area is severely threatened by diseases caused by bacterial pathogens, and Aeromonas veronii has been the main pathogen in recent years. In this paper, ERIC-PCR genotyping, virulence gene and antimicrobial resistance gene detection, and drug susceptibility tests were carried out on 46 A. veronii isolates obtained from aquaculture systems in the Poyang Lake area from 2016 to 2020. The results showed that the A. veronii strains in the Poyang Lake area had high genetic diversity, and 46 strains produced 36 ERIC genotypes. There were no geographical and temporal differences in the cluster analysis results and no dominant clones. All 13 virulence genes tested were detected, and all isolates had harbored 2 or more virulence genes, with a maximum of 12 virulence genes detected. Among the 22 antimicrobial resistance genes selected, 15 were detected; 97.8% of the isolates contained 2 or more antimicrobial resistance genes, with a maximum of 9 antimicrobial resistance genes. Drug susceptibility tests showed that some strains were resistant to a variety of traditionally effective drugs for Aeromomas, such as enrofloxacin and florfenicol. This study provides a reference for exploring the impact of aquaculture in the Poyang Lake area on public health.
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spelling pubmed-97910642022-12-27 Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China Xu, Xiandong Fu, Huiyun Wan, Guoyuan Huang, Jiangfeng Zhou, Zhiyong Rao, Yi Liu, Lihui Wen, Chungen Front Microbiol Microbiology The area around Poyang Lake is the main aquaculture area in Jiangxi Province, China, and an important base for the supply of freshwater aquatic products. Aquaculture in the Poyang Lake area is severely threatened by diseases caused by bacterial pathogens, and Aeromonas veronii has been the main pathogen in recent years. In this paper, ERIC-PCR genotyping, virulence gene and antimicrobial resistance gene detection, and drug susceptibility tests were carried out on 46 A. veronii isolates obtained from aquaculture systems in the Poyang Lake area from 2016 to 2020. The results showed that the A. veronii strains in the Poyang Lake area had high genetic diversity, and 46 strains produced 36 ERIC genotypes. There were no geographical and temporal differences in the cluster analysis results and no dominant clones. All 13 virulence genes tested were detected, and all isolates had harbored 2 or more virulence genes, with a maximum of 12 virulence genes detected. Among the 22 antimicrobial resistance genes selected, 15 were detected; 97.8% of the isolates contained 2 or more antimicrobial resistance genes, with a maximum of 9 antimicrobial resistance genes. Drug susceptibility tests showed that some strains were resistant to a variety of traditionally effective drugs for Aeromomas, such as enrofloxacin and florfenicol. This study provides a reference for exploring the impact of aquaculture in the Poyang Lake area on public health. Frontiers Media S.A. 2022-12-12 /pmc/articles/PMC9791064/ /pubmed/36578578 http://dx.doi.org/10.3389/fmicb.2022.1042007 Text en Copyright © 2022 Xu, Fu, Wan, Huang, Zhou, Rao, Liu and Wen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Xiandong
Fu, Huiyun
Wan, Guoyuan
Huang, Jiangfeng
Zhou, Zhiyong
Rao, Yi
Liu, Lihui
Wen, Chungen
Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title_full Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title_fullStr Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title_full_unstemmed Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title_short Prevalence and genetic diversity of Aeromonas veronii isolated from aquaculture systems in the Poyang Lake area, China
title_sort prevalence and genetic diversity of aeromonas veronii isolated from aquaculture systems in the poyang lake area, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791064/
https://www.ncbi.nlm.nih.gov/pubmed/36578578
http://dx.doi.org/10.3389/fmicb.2022.1042007
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