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Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana

Macrophomina root rot disease (MRRD) caused by Macrophomina phaseolina is an emerging threat to the profitable cowpea production in northern Ghana. Recommended control methods including the use of fungicides are ineffective and expensive for resource poor farmers whilst biocontrol options are not co...

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Autores principales: Lamini, Salim, Kusi, Francis, Cornelius, Eric William, Danquah, Agyemang, Attamah, Patrick, Mukhtaru, Zakaria, Awuku, Frederick Justice, Owusu, Emmanuel Yaw, Acheampong, Mavis, Mensah, Gloria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791353/
https://www.ncbi.nlm.nih.gov/pubmed/36578397
http://dx.doi.org/10.1016/j.heliyon.2022.e12217
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author Lamini, Salim
Kusi, Francis
Cornelius, Eric William
Danquah, Agyemang
Attamah, Patrick
Mukhtaru, Zakaria
Awuku, Frederick Justice
Owusu, Emmanuel Yaw
Acheampong, Mavis
Mensah, Gloria
author_facet Lamini, Salim
Kusi, Francis
Cornelius, Eric William
Danquah, Agyemang
Attamah, Patrick
Mukhtaru, Zakaria
Awuku, Frederick Justice
Owusu, Emmanuel Yaw
Acheampong, Mavis
Mensah, Gloria
author_sort Lamini, Salim
collection PubMed
description Macrophomina root rot disease (MRRD) caused by Macrophomina phaseolina is an emerging threat to the profitable cowpea production in northern Ghana. Recommended control methods including the use of fungicides are ineffective and expensive for resource poor farmers whilst biocontrol options are not commercially available. An integrated method based on host plant resistance is considered the cheapest and most effective method of managing the disease. This study sought to confirm and characterize previously identified MRRD isolates from Northern Ghana using molecular technology, and to identify cowpea with potential sources of resistance to the MRRD. A PCR assay of ten isolates of the cowpea root rot pathogen revealed all isolates belonged to the species M. phaseolina, whilst a nucleotide BLAST of eight isolates showed 98% similarity with the sequences of Macrophomina isolates from other host available in GenBank. A sick pot method evaluation of 49 cowpea lines found 10 lines resistant to MRRD on a 1–9 disease severity scale (disease score, less than 5). A selection of eight resistant lines (Suvita 2, Abagbaala, IT97K573-1-1, IT93K-503-1-1, Hewale, AV2 3224, Nhyira and T2T4), and a susceptible check (Songotra) were evaluated against 10 isolates of M. phaseolina using a sick pot method. All the genotypes except for the susceptible check were resistant to MRRD. Thus, these genotypes could be used in cowpea MRRD resistance breeding programs.
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spelling pubmed-97913532022-12-27 Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana Lamini, Salim Kusi, Francis Cornelius, Eric William Danquah, Agyemang Attamah, Patrick Mukhtaru, Zakaria Awuku, Frederick Justice Owusu, Emmanuel Yaw Acheampong, Mavis Mensah, Gloria Heliyon Research Article Macrophomina root rot disease (MRRD) caused by Macrophomina phaseolina is an emerging threat to the profitable cowpea production in northern Ghana. Recommended control methods including the use of fungicides are ineffective and expensive for resource poor farmers whilst biocontrol options are not commercially available. An integrated method based on host plant resistance is considered the cheapest and most effective method of managing the disease. This study sought to confirm and characterize previously identified MRRD isolates from Northern Ghana using molecular technology, and to identify cowpea with potential sources of resistance to the MRRD. A PCR assay of ten isolates of the cowpea root rot pathogen revealed all isolates belonged to the species M. phaseolina, whilst a nucleotide BLAST of eight isolates showed 98% similarity with the sequences of Macrophomina isolates from other host available in GenBank. A sick pot method evaluation of 49 cowpea lines found 10 lines resistant to MRRD on a 1–9 disease severity scale (disease score, less than 5). A selection of eight resistant lines (Suvita 2, Abagbaala, IT97K573-1-1, IT93K-503-1-1, Hewale, AV2 3224, Nhyira and T2T4), and a susceptible check (Songotra) were evaluated against 10 isolates of M. phaseolina using a sick pot method. All the genotypes except for the susceptible check were resistant to MRRD. Thus, these genotypes could be used in cowpea MRRD resistance breeding programs. Elsevier 2022-12-10 /pmc/articles/PMC9791353/ /pubmed/36578397 http://dx.doi.org/10.1016/j.heliyon.2022.e12217 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Lamini, Salim
Kusi, Francis
Cornelius, Eric William
Danquah, Agyemang
Attamah, Patrick
Mukhtaru, Zakaria
Awuku, Frederick Justice
Owusu, Emmanuel Yaw
Acheampong, Mavis
Mensah, Gloria
Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title_full Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title_fullStr Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title_full_unstemmed Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title_short Identification of sources of resistance in cowpea lines to Macrophomina root rot disease in Northern Ghana
title_sort identification of sources of resistance in cowpea lines to macrophomina root rot disease in northern ghana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791353/
https://www.ncbi.nlm.nih.gov/pubmed/36578397
http://dx.doi.org/10.1016/j.heliyon.2022.e12217
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