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Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos

PURPOSE: Recently, ultrahigh-dose-rate radiation therapy (UHDR-RT) has emerged as a promising strategy to increase the benefit/risk ratio of external RT. Extensive work is on the way to characterize the physical and biological parameters that control the so-called “Flash” effect. However, this healt...

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Autores principales: Saade, Gaëlle, Bogaerts, Eva, Chiavassa, Sophie, Blain, Guillaume, Delpon, Grégory, Evin, Manon, Ghannam, Youssef, Haddad, Ferid, Haustermans, Karin, Koumeir, Charbel, Macaeva, Ellina, Maigne, Lydia, Mouchard, Quentin, Servagent, Noël, Sterpin, Edmond, Supiot, Stéphane, Potiron, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791798/
https://www.ncbi.nlm.nih.gov/pubmed/36578276
http://dx.doi.org/10.1016/j.adro.2022.101124
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author Saade, Gaëlle
Bogaerts, Eva
Chiavassa, Sophie
Blain, Guillaume
Delpon, Grégory
Evin, Manon
Ghannam, Youssef
Haddad, Ferid
Haustermans, Karin
Koumeir, Charbel
Macaeva, Ellina
Maigne, Lydia
Mouchard, Quentin
Servagent, Noël
Sterpin, Edmond
Supiot, Stéphane
Potiron, Vincent
author_facet Saade, Gaëlle
Bogaerts, Eva
Chiavassa, Sophie
Blain, Guillaume
Delpon, Grégory
Evin, Manon
Ghannam, Youssef
Haddad, Ferid
Haustermans, Karin
Koumeir, Charbel
Macaeva, Ellina
Maigne, Lydia
Mouchard, Quentin
Servagent, Noël
Sterpin, Edmond
Supiot, Stéphane
Potiron, Vincent
author_sort Saade, Gaëlle
collection PubMed
description PURPOSE: Recently, ultrahigh-dose-rate radiation therapy (UHDR-RT) has emerged as a promising strategy to increase the benefit/risk ratio of external RT. Extensive work is on the way to characterize the physical and biological parameters that control the so-called “Flash” effect. However, this healthy/tumor differential effect is observable in in vivo models, which thereby drastically limits the amount of work that is achievable in a timely manner. METHODS AND MATERIALS: In this study, zebrafish embryos were used to compare the effect of UHDR irradiation (8-9 kGy/s) to conventional RT dose rate (0.2 Gy/s) with a 68 MeV proton beam. Viability, body length, spine curvature, and pericardial edema were measured 4 days postirradiation. RESULTS: We show that body length is significantly greater after UHDR-RT compared with conventional RT by 180 µm at 30 Gy and 90 µm at 40 Gy, while pericardial edema is only reduced at 30 Gy. No differences were obtained in terms of survival or spine curvature. CONCLUSIONS: Zebrafish embryo length appears as a robust endpoint, and we anticipate that this model will substantially fasten the study of UHDR proton-beam parameters necessary for “Flash.”
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spelling pubmed-97917982022-12-27 Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos Saade, Gaëlle Bogaerts, Eva Chiavassa, Sophie Blain, Guillaume Delpon, Grégory Evin, Manon Ghannam, Youssef Haddad, Ferid Haustermans, Karin Koumeir, Charbel Macaeva, Ellina Maigne, Lydia Mouchard, Quentin Servagent, Noël Sterpin, Edmond Supiot, Stéphane Potiron, Vincent Adv Radiat Oncol Biology Contribution PURPOSE: Recently, ultrahigh-dose-rate radiation therapy (UHDR-RT) has emerged as a promising strategy to increase the benefit/risk ratio of external RT. Extensive work is on the way to characterize the physical and biological parameters that control the so-called “Flash” effect. However, this healthy/tumor differential effect is observable in in vivo models, which thereby drastically limits the amount of work that is achievable in a timely manner. METHODS AND MATERIALS: In this study, zebrafish embryos were used to compare the effect of UHDR irradiation (8-9 kGy/s) to conventional RT dose rate (0.2 Gy/s) with a 68 MeV proton beam. Viability, body length, spine curvature, and pericardial edema were measured 4 days postirradiation. RESULTS: We show that body length is significantly greater after UHDR-RT compared with conventional RT by 180 µm at 30 Gy and 90 µm at 40 Gy, while pericardial edema is only reduced at 30 Gy. No differences were obtained in terms of survival or spine curvature. CONCLUSIONS: Zebrafish embryo length appears as a robust endpoint, and we anticipate that this model will substantially fasten the study of UHDR proton-beam parameters necessary for “Flash.” Elsevier 2022-11-19 /pmc/articles/PMC9791798/ /pubmed/36578276 http://dx.doi.org/10.1016/j.adro.2022.101124 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biology Contribution
Saade, Gaëlle
Bogaerts, Eva
Chiavassa, Sophie
Blain, Guillaume
Delpon, Grégory
Evin, Manon
Ghannam, Youssef
Haddad, Ferid
Haustermans, Karin
Koumeir, Charbel
Macaeva, Ellina
Maigne, Lydia
Mouchard, Quentin
Servagent, Noël
Sterpin, Edmond
Supiot, Stéphane
Potiron, Vincent
Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title_full Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title_fullStr Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title_full_unstemmed Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title_short Ultrahigh-Dose-Rate Proton Irradiation Elicits Reduced Toxicity in Zebrafish Embryos
title_sort ultrahigh-dose-rate proton irradiation elicits reduced toxicity in zebrafish embryos
topic Biology Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791798/
https://www.ncbi.nlm.nih.gov/pubmed/36578276
http://dx.doi.org/10.1016/j.adro.2022.101124
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