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Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335
Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb; pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a complete MPF(T) (previously type IVA secretion system) and a functional o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791958/ https://www.ncbi.nlm.nih.gov/pubmed/36578579 http://dx.doi.org/10.3389/fmicb.2022.1076710 |
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author | Añorga, Maite Urriza, Miriam Ramos, Cayo Murillo, Jesús |
author_facet | Añorga, Maite Urriza, Miriam Ramos, Cayo Murillo, Jesús |
author_sort | Añorga, Maite |
collection | PubMed |
description | Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb; pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a complete MPF(T) (previously type IVA secretion system) and a functional origin of conjugational transfer adjacent to a relaxase of the MOB(P) family; pC also contains a functional oriT-MOB(P) array, whereas pA contains an incomplete MPF(I) (previously type IVB secretion system), but not a recognizable oriT. Plasmid transfer occurred on solid and in liquid media, and on leaf surfaces of a non-host plant (Phaseolus vulgaris) with high (pB) or moderate frequency (pC); pA was transferred only occasionally after cointegration with pB. We found three plasmid-borne and three chromosomal relaxase genes, although the chromosomal relaxases did not contribute to plasmid dissemination. The MOB(P) relaxase genes of pB and pC were functionally interchangeable, although with differing efficiencies. We also identified a functional MOB(Q) mobilization region in pC, which could only mobilize this plasmid. Plasmid pB could be efficiently transferred to strains of six phylogroups of P. syringae sensu lato, whereas pC could only be mobilized to two strains of phylogroup 3 (genomospecies 2). In two of the recipient strains, pB was stably maintained after 21 subcultures in liquid medium. The carriage of several relaxases by the native plasmids of P. syringae impacts their transfer frequency and, by providing functional diversity and redundancy, adds robustness to the conjugation system. |
format | Online Article Text |
id | pubmed-9791958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97919582022-12-27 Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 Añorga, Maite Urriza, Miriam Ramos, Cayo Murillo, Jesús Front Microbiol Microbiology Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb; pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a complete MPF(T) (previously type IVA secretion system) and a functional origin of conjugational transfer adjacent to a relaxase of the MOB(P) family; pC also contains a functional oriT-MOB(P) array, whereas pA contains an incomplete MPF(I) (previously type IVB secretion system), but not a recognizable oriT. Plasmid transfer occurred on solid and in liquid media, and on leaf surfaces of a non-host plant (Phaseolus vulgaris) with high (pB) or moderate frequency (pC); pA was transferred only occasionally after cointegration with pB. We found three plasmid-borne and three chromosomal relaxase genes, although the chromosomal relaxases did not contribute to plasmid dissemination. The MOB(P) relaxase genes of pB and pC were functionally interchangeable, although with differing efficiencies. We also identified a functional MOB(Q) mobilization region in pC, which could only mobilize this plasmid. Plasmid pB could be efficiently transferred to strains of six phylogroups of P. syringae sensu lato, whereas pC could only be mobilized to two strains of phylogroup 3 (genomospecies 2). In two of the recipient strains, pB was stably maintained after 21 subcultures in liquid medium. The carriage of several relaxases by the native plasmids of P. syringae impacts their transfer frequency and, by providing functional diversity and redundancy, adds robustness to the conjugation system. Frontiers Media S.A. 2022-12-12 /pmc/articles/PMC9791958/ /pubmed/36578579 http://dx.doi.org/10.3389/fmicb.2022.1076710 Text en Copyright © 2022 Añorga, Urriza, Ramos and Murillo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Añorga, Maite Urriza, Miriam Ramos, Cayo Murillo, Jesús Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title | Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title_full | Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title_fullStr | Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title_full_unstemmed | Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title_short | Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335 |
title_sort | multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium pseudomonas syringae pv. savastanoi ncppb 3335 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791958/ https://www.ncbi.nlm.nih.gov/pubmed/36578579 http://dx.doi.org/10.3389/fmicb.2022.1076710 |
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