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Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits
Eimeria magna is a common pathogen in rabbits, which results in lethargy, weight loss, diarrhea, and even death in severe cases after infection. The current method for preventing rabbit coccidiosis is to add anticoccidial drugs to the diet. However, there are many concerns about drug resistance and...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792316/ https://www.ncbi.nlm.nih.gov/pubmed/36572833 http://dx.doi.org/10.1007/s00436-022-07774-3 |
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author | Chen, Hao Pu, Jiayan Xiao, Jie Bai, Xin Zheng, Ruoyu Gu, Xiaobin Xie, Yue He, Ran Xu, Jing Jing, Bo Peng, Xuerong Ren, Yongjun Yang, Guangyou |
author_facet | Chen, Hao Pu, Jiayan Xiao, Jie Bai, Xin Zheng, Ruoyu Gu, Xiaobin Xie, Yue He, Ran Xu, Jing Jing, Bo Peng, Xuerong Ren, Yongjun Yang, Guangyou |
author_sort | Chen, Hao |
collection | PubMed |
description | Eimeria magna is a common pathogen in rabbits, which results in lethargy, weight loss, diarrhea, and even death in severe cases after infection. The current method for preventing rabbit coccidiosis is to add anticoccidial drugs to the diet. However, there are many concerns about drug resistance and drug residues. In our study, the rEmMIC2 and rEmMIC3 proteins were cloned and expressed to evaluate potential as recombinant subunit vaccine candidate antigens. The protective effects of rEmMIC2 and rEmMIC3 were evaluated by the relative weight gain ratio, oocyst decrease rate, anticoccidial index, feed conversion ratio, pathological alterations, clinical symptoms, specific IgG antibody, and cytokine levels in rabbits. The molecular weights of rEmMIC2 and rEmMIC3 were 18.69 kDa and 17.47 kDa, respectively. After the coccidia challenge, the control groups showed anorexia and soft poop, whereas the experimental group showed few anorexia symptoms. Significantly different from the control group, the relative weight gain ratios of the immunized rEmMIC2 and rEmMIC3 groups were 78.37% and 75.29%, respectively, and the oocyst reduction was 77.95% and 76.09%, respectively, and the anticoccidial index was 171.12 and 169.29, respectively. IgG antibody, IFN-γ, IL-4, IL-10, and IL-17 levels were significantly increased in the experimental group. The results showed that rEmMIC2 and rEmMIC3 have potential as vaccine candidate antigens. |
format | Online Article Text |
id | pubmed-9792316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-97923162022-12-27 Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits Chen, Hao Pu, Jiayan Xiao, Jie Bai, Xin Zheng, Ruoyu Gu, Xiaobin Xie, Yue He, Ran Xu, Jing Jing, Bo Peng, Xuerong Ren, Yongjun Yang, Guangyou Parasitol Res Research Eimeria magna is a common pathogen in rabbits, which results in lethargy, weight loss, diarrhea, and even death in severe cases after infection. The current method for preventing rabbit coccidiosis is to add anticoccidial drugs to the diet. However, there are many concerns about drug resistance and drug residues. In our study, the rEmMIC2 and rEmMIC3 proteins were cloned and expressed to evaluate potential as recombinant subunit vaccine candidate antigens. The protective effects of rEmMIC2 and rEmMIC3 were evaluated by the relative weight gain ratio, oocyst decrease rate, anticoccidial index, feed conversion ratio, pathological alterations, clinical symptoms, specific IgG antibody, and cytokine levels in rabbits. The molecular weights of rEmMIC2 and rEmMIC3 were 18.69 kDa and 17.47 kDa, respectively. After the coccidia challenge, the control groups showed anorexia and soft poop, whereas the experimental group showed few anorexia symptoms. Significantly different from the control group, the relative weight gain ratios of the immunized rEmMIC2 and rEmMIC3 groups were 78.37% and 75.29%, respectively, and the oocyst reduction was 77.95% and 76.09%, respectively, and the anticoccidial index was 171.12 and 169.29, respectively. IgG antibody, IFN-γ, IL-4, IL-10, and IL-17 levels were significantly increased in the experimental group. The results showed that rEmMIC2 and rEmMIC3 have potential as vaccine candidate antigens. Springer Berlin Heidelberg 2022-12-27 2023 /pmc/articles/PMC9792316/ /pubmed/36572833 http://dx.doi.org/10.1007/s00436-022-07774-3 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Chen, Hao Pu, Jiayan Xiao, Jie Bai, Xin Zheng, Ruoyu Gu, Xiaobin Xie, Yue He, Ran Xu, Jing Jing, Bo Peng, Xuerong Ren, Yongjun Yang, Guangyou Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title | Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title_full | Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title_fullStr | Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title_full_unstemmed | Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title_short | Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits |
title_sort | evaluation of the immune protective effects of remmic2 and remmic3 from eimeria magna in rabbits |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792316/ https://www.ncbi.nlm.nih.gov/pubmed/36572833 http://dx.doi.org/10.1007/s00436-022-07774-3 |
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