Multiplexed evaluation of mouse wound tissue using oligonucleotide barcoding with single-cell RNA sequencing

Despite its rapidly increased availability for the study of complex tissue, single-cell RNA sequencing remains prohibitively expensive for large studies. Here, we present a protocol using oligonucleotide barcoding for the tagging and pooling of multiple samples from healing wounds, which are among t...

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Detalles Bibliográficos
Autores principales: Januszyk, Michael, Griffin, Michelle, Mascharak, Shamik, Talbott, Heather E., Chen, Kellen, Henn, Dominic, Spielman, Amanda F., Parker, Jennifer B.L., Liang, Norah E., Cotterell, Asha, Guardino, Nicholas, Foster, Deshka S., Wagh, Dhananjay, Coller, John, Gurtner, Geoffrey C., Wan, Derrick C., Longaker, Michael T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792534/
https://www.ncbi.nlm.nih.gov/pubmed/36525348
http://dx.doi.org/10.1016/j.xpro.2022.101946

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