A flow-cytometry-based assay to assess granule exocytosis and GZB delivery by human CD8 T cells and NK cells

CD8 T and NK cells mediate killing by delivery of perforin and granzyme B (GZB) stored in lysosome-like granules. We present a flow-cytometry-based protocol combined with a redirected killing assay to evaluate granule exocytosis and the cytotoxic potential of human CD8 T cells and NK cells. We descr...

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Detalles Bibliográficos
Autores principales: Li, Tong, Smith, Mindy, Abdussamad, Maryam, Katz, Grace, Catalfamo, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792553/
https://www.ncbi.nlm.nih.gov/pubmed/36527713
http://dx.doi.org/10.1016/j.xpro.2022.101939

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