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Isolation of adoptively transferred CD8(+) T cells in mouse tumor tissues for lipid peroxidation detection
The lipid peroxidation level of tumor-infiltrating CD8(+) T cells is crucial for its activity and longevity. Here, we describe a protocol for effective and epitope-preserving dissociation of mouse tumors and subsequent leukocyte purification and lipid peroxidation staining of adoptively transferred...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792557/ https://www.ncbi.nlm.nih.gov/pubmed/36525345 http://dx.doi.org/10.1016/j.xpro.2022.101945 |