Generation of germ cell-deficient pigs by NANOS3 knockout

NANOS3 is an evolutionarily conserved gene expressed in primordial germ cells that is important for germ cell development. Germ cell deletion by NANOS3 knockout has been reported in several mammalian species, but its function in pigs is unclear. In the present study, we investigated the germline eff...

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Autores principales: KOGASAKA, Yuhei, MURAKAMI, Sho, YAMASHITA, Shiro, KIMURA, Daisuke, FURUMOTO, Yoshinori, IGUCHI, Kana, SENDAI, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792658/
https://www.ncbi.nlm.nih.gov/pubmed/36273893
http://dx.doi.org/10.1262/jrd.2022-028
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author KOGASAKA, Yuhei
MURAKAMI, Sho
YAMASHITA, Shiro
KIMURA, Daisuke
FURUMOTO, Yoshinori
IGUCHI, Kana
SENDAI, Yutaka
author_facet KOGASAKA, Yuhei
MURAKAMI, Sho
YAMASHITA, Shiro
KIMURA, Daisuke
FURUMOTO, Yoshinori
IGUCHI, Kana
SENDAI, Yutaka
author_sort KOGASAKA, Yuhei
collection PubMed
description NANOS3 is an evolutionarily conserved gene expressed in primordial germ cells that is important for germ cell development. Germ cell deletion by NANOS3 knockout has been reported in several mammalian species, but its function in pigs is unclear. In the present study, we investigated the germline effects of NANOS3 knockout in pigs using CRISPR/Cas9. Embryo transfer of CRISPR/Cas9-modified embryos produced ten offspring, of which one showed wild-type NANOS3 alleles, eight had two mutant NANOS3 alleles, and the other exhibited mosaicism (four mutant alleles). Histological analysis revealed no germ cells in the testes or ovaries of any of the nine mutant pigs. These results demonstrated that NANOS3 is crucial for porcine germ cell production.
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spelling pubmed-97926582022-12-28 Generation of germ cell-deficient pigs by NANOS3 knockout KOGASAKA, Yuhei MURAKAMI, Sho YAMASHITA, Shiro KIMURA, Daisuke FURUMOTO, Yoshinori IGUCHI, Kana SENDAI, Yutaka J Reprod Dev Original Article NANOS3 is an evolutionarily conserved gene expressed in primordial germ cells that is important for germ cell development. Germ cell deletion by NANOS3 knockout has been reported in several mammalian species, but its function in pigs is unclear. In the present study, we investigated the germline effects of NANOS3 knockout in pigs using CRISPR/Cas9. Embryo transfer of CRISPR/Cas9-modified embryos produced ten offspring, of which one showed wild-type NANOS3 alleles, eight had two mutant NANOS3 alleles, and the other exhibited mosaicism (four mutant alleles). Histological analysis revealed no germ cells in the testes or ovaries of any of the nine mutant pigs. These results demonstrated that NANOS3 is crucial for porcine germ cell production. The Society for Reproduction and Development 2022-10-23 2022-12 /pmc/articles/PMC9792658/ /pubmed/36273893 http://dx.doi.org/10.1262/jrd.2022-028 Text en ©2022 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
KOGASAKA, Yuhei
MURAKAMI, Sho
YAMASHITA, Shiro
KIMURA, Daisuke
FURUMOTO, Yoshinori
IGUCHI, Kana
SENDAI, Yutaka
Generation of germ cell-deficient pigs by NANOS3 knockout
title Generation of germ cell-deficient pigs by NANOS3 knockout
title_full Generation of germ cell-deficient pigs by NANOS3 knockout
title_fullStr Generation of germ cell-deficient pigs by NANOS3 knockout
title_full_unstemmed Generation of germ cell-deficient pigs by NANOS3 knockout
title_short Generation of germ cell-deficient pigs by NANOS3 knockout
title_sort generation of germ cell-deficient pigs by nanos3 knockout
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792658/
https://www.ncbi.nlm.nih.gov/pubmed/36273893
http://dx.doi.org/10.1262/jrd.2022-028
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