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Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays

For rapid and sensitive pathogen screening from field outbreaks, molecular techniques such as qPCR-based simultaneous detections are efficient. Respiratory diseases are the most detrimental diseases to the poultry industry and need to be addressed because of their major economic losses. In the curre...

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Autores principales: Parvin, Rokshana, Kabiraj, Congriev Kumar, Hossain, Ismail, Hassan, Alamgir, Begum, Jahan Ara, Nooruzzaman, Mohammed, Islam, Md. Taohidul, Chowdhury, Emdadul Haque
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792859/
https://www.ncbi.nlm.nih.gov/pubmed/36583036
http://dx.doi.org/10.3389/fvets.2022.1036757
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author Parvin, Rokshana
Kabiraj, Congriev Kumar
Hossain, Ismail
Hassan, Alamgir
Begum, Jahan Ara
Nooruzzaman, Mohammed
Islam, Md. Taohidul
Chowdhury, Emdadul Haque
author_facet Parvin, Rokshana
Kabiraj, Congriev Kumar
Hossain, Ismail
Hassan, Alamgir
Begum, Jahan Ara
Nooruzzaman, Mohammed
Islam, Md. Taohidul
Chowdhury, Emdadul Haque
author_sort Parvin, Rokshana
collection PubMed
description For rapid and sensitive pathogen screening from field outbreaks, molecular techniques such as qPCR-based simultaneous detections are efficient. Respiratory diseases are the most detrimental diseases to the poultry industry and need to be addressed because of their major economic losses. In the current study, we have applied two different detection assays: one for simultaneous detection of avian influenza virus (AIV; M gene) and subtyping (H5, N1, H9, N2) using TaqMan probe chemistry (TaqMan multitarget) and another for simultaneous detection of Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and infectious laryngotracheitis virus (ILTV) using SYBR Green chemistry (SYBR Green multitarget). Two individual qPCRs were conducted for the detection of four pathogens. Surveillance of tissue (n = 158) and oropharyngeal swab (206) samples from multiple poultry flocks during the years April 2020–July 2022 applying the TaqMan and SYBR Green multitarget qPCRs revealed that 48.9% of samples were positive for respiratory infections, of which 17.2% were positive for NDV, 25.5% were positive for AIV, 9.9% were positive for IBV, and only a single positive (0.3%) for ILTV. Among the AIV, 35% were highly pathogenic subtype H5N1 and 65% were low pathogenic subtype H9N2. Co-infections of 2–3 respiratory viruses were also accurately detected. Respiratory viral pathogens are quite common in Bangladeshi poultry and can be successfully detected using multitarget simultaneous real-time quantitative polymerase chain reaction (RT-qPCR) assays like those adopted in the current study. Increased mass surveillance, along with the molecular characterization of the circulating respiratory viruses, is crucial to control the epidemic and subsequently save the Bangladeshi poultry industry.
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spelling pubmed-97928592022-12-28 Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays Parvin, Rokshana Kabiraj, Congriev Kumar Hossain, Ismail Hassan, Alamgir Begum, Jahan Ara Nooruzzaman, Mohammed Islam, Md. Taohidul Chowdhury, Emdadul Haque Front Vet Sci Veterinary Science For rapid and sensitive pathogen screening from field outbreaks, molecular techniques such as qPCR-based simultaneous detections are efficient. Respiratory diseases are the most detrimental diseases to the poultry industry and need to be addressed because of their major economic losses. In the current study, we have applied two different detection assays: one for simultaneous detection of avian influenza virus (AIV; M gene) and subtyping (H5, N1, H9, N2) using TaqMan probe chemistry (TaqMan multitarget) and another for simultaneous detection of Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and infectious laryngotracheitis virus (ILTV) using SYBR Green chemistry (SYBR Green multitarget). Two individual qPCRs were conducted for the detection of four pathogens. Surveillance of tissue (n = 158) and oropharyngeal swab (206) samples from multiple poultry flocks during the years April 2020–July 2022 applying the TaqMan and SYBR Green multitarget qPCRs revealed that 48.9% of samples were positive for respiratory infections, of which 17.2% were positive for NDV, 25.5% were positive for AIV, 9.9% were positive for IBV, and only a single positive (0.3%) for ILTV. Among the AIV, 35% were highly pathogenic subtype H5N1 and 65% were low pathogenic subtype H9N2. Co-infections of 2–3 respiratory viruses were also accurately detected. Respiratory viral pathogens are quite common in Bangladeshi poultry and can be successfully detected using multitarget simultaneous real-time quantitative polymerase chain reaction (RT-qPCR) assays like those adopted in the current study. Increased mass surveillance, along with the molecular characterization of the circulating respiratory viruses, is crucial to control the epidemic and subsequently save the Bangladeshi poultry industry. Frontiers Media S.A. 2022-12-13 /pmc/articles/PMC9792859/ /pubmed/36583036 http://dx.doi.org/10.3389/fvets.2022.1036757 Text en Copyright © 2022 Parvin, Kabiraj, Hossain, Hassan, Begum, Nooruzzaman, Islam and Chowdhury. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Parvin, Rokshana
Kabiraj, Congriev Kumar
Hossain, Ismail
Hassan, Alamgir
Begum, Jahan Ara
Nooruzzaman, Mohammed
Islam, Md. Taohidul
Chowdhury, Emdadul Haque
Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title_full Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title_fullStr Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title_full_unstemmed Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title_short Investigation of respiratory disease outbreaks of poultry in Bangladesh using two real-time PCR-based simultaneous detection assays
title_sort investigation of respiratory disease outbreaks of poultry in bangladesh using two real-time pcr-based simultaneous detection assays
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792859/
https://www.ncbi.nlm.nih.gov/pubmed/36583036
http://dx.doi.org/10.3389/fvets.2022.1036757
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