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Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation
Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid different...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792907/ https://www.ncbi.nlm.nih.gov/pubmed/36582828 http://dx.doi.org/10.1016/j.isci.2022.105739 |
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author | Bouthelier, Antonio Fernández-Arroyo, Lucía Mesa-Ciller, Claudia Cibrian, Danay Martín-Cófreces, Noa Beatriz Castillo-González, Raquel Calero, Macarena Herráez-Aguilar, Diego Guajardo-Grence, Andrea Pacheco, Ana María Marcos-Jiménez, Ana Quiroga, Borja Morado, Marta Monroy, Francisco Muñoz-Calleja, Cecilia Sánchez-Madrid, Francisco Urrutia, Andrés A. Aragonés, Julián |
author_facet | Bouthelier, Antonio Fernández-Arroyo, Lucía Mesa-Ciller, Claudia Cibrian, Danay Martín-Cófreces, Noa Beatriz Castillo-González, Raquel Calero, Macarena Herráez-Aguilar, Diego Guajardo-Grence, Andrea Pacheco, Ana María Marcos-Jiménez, Ana Quiroga, Borja Morado, Marta Monroy, Francisco Muñoz-Calleja, Cecilia Sánchez-Madrid, Francisco Urrutia, Andrés A. Aragonés, Julián |
author_sort | Bouthelier, Antonio |
collection | PubMed |
description | Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs. |
format | Online Article Text |
id | pubmed-9792907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97929072022-12-28 Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation Bouthelier, Antonio Fernández-Arroyo, Lucía Mesa-Ciller, Claudia Cibrian, Danay Martín-Cófreces, Noa Beatriz Castillo-González, Raquel Calero, Macarena Herráez-Aguilar, Diego Guajardo-Grence, Andrea Pacheco, Ana María Marcos-Jiménez, Ana Quiroga, Borja Morado, Marta Monroy, Francisco Muñoz-Calleja, Cecilia Sánchez-Madrid, Francisco Urrutia, Andrés A. Aragonés, Julián iScience Article Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs. Elsevier 2022-12-05 /pmc/articles/PMC9792907/ /pubmed/36582828 http://dx.doi.org/10.1016/j.isci.2022.105739 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Bouthelier, Antonio Fernández-Arroyo, Lucía Mesa-Ciller, Claudia Cibrian, Danay Martín-Cófreces, Noa Beatriz Castillo-González, Raquel Calero, Macarena Herráez-Aguilar, Diego Guajardo-Grence, Andrea Pacheco, Ana María Marcos-Jiménez, Ana Quiroga, Borja Morado, Marta Monroy, Francisco Muñoz-Calleja, Cecilia Sánchez-Madrid, Francisco Urrutia, Andrés A. Aragonés, Julián Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title | Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title_full | Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title_fullStr | Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title_full_unstemmed | Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title_short | Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation |
title_sort | erythroid slc7a5/slc3a2 amino acid carrier controls red blood cell size and maturation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792907/ https://www.ncbi.nlm.nih.gov/pubmed/36582828 http://dx.doi.org/10.1016/j.isci.2022.105739 |
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