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Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase

Anaplasma phagocytophilum modulates various cell signaling pathways in mammalian cells for its survival. In this study, we report that A. phagocytophilum modulates tick tryptophan pathway to activate arthropod p38 MAP kinase for the survival of both this bacterium and its vector host. Increased leve...

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Autores principales: Namjoshi, Prachi, Dahmani, Mustapha, Sultana, Hameeda, Neelakanta, Girish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792911/
https://www.ncbi.nlm.nih.gov/pubmed/36582833
http://dx.doi.org/10.1016/j.isci.2022.105730
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author Namjoshi, Prachi
Dahmani, Mustapha
Sultana, Hameeda
Neelakanta, Girish
author_facet Namjoshi, Prachi
Dahmani, Mustapha
Sultana, Hameeda
Neelakanta, Girish
author_sort Namjoshi, Prachi
collection PubMed
description Anaplasma phagocytophilum modulates various cell signaling pathways in mammalian cells for its survival. In this study, we report that A. phagocytophilum modulates tick tryptophan pathway to activate arthropod p38 MAP kinase for the survival of both this bacterium and its vector host. Increased level of tryptophan metabolite, xanthurenic acid (XA), was evident in A. phagocytophilum-infected ticks and tick cells. Lower levels of cell death markers and increased levels of total and phosphorylated p38 MAPK was noted in A. phagocytophilum-infected ticks and tick cells. Treatment with XA increased phosphorylated p38 MAPK levels and reduced cell death in A. phagocytophilum-infected tick cells. Furthermore, treatment with p38 MAPK inhibitor affected bacterial replication, decreased phosphorylated p38 MAPK levels and increased tick cell death. However, XA reversed these effects. Taken together, we provide evidence that rickettsial pathogen modulates arthropod tryptophan and p38 MAPK pathways to inhibit cell death for its survival in ticks.
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spelling pubmed-97929112022-12-28 Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase Namjoshi, Prachi Dahmani, Mustapha Sultana, Hameeda Neelakanta, Girish iScience Article Anaplasma phagocytophilum modulates various cell signaling pathways in mammalian cells for its survival. In this study, we report that A. phagocytophilum modulates tick tryptophan pathway to activate arthropod p38 MAP kinase for the survival of both this bacterium and its vector host. Increased level of tryptophan metabolite, xanthurenic acid (XA), was evident in A. phagocytophilum-infected ticks and tick cells. Lower levels of cell death markers and increased levels of total and phosphorylated p38 MAPK was noted in A. phagocytophilum-infected ticks and tick cells. Treatment with XA increased phosphorylated p38 MAPK levels and reduced cell death in A. phagocytophilum-infected tick cells. Furthermore, treatment with p38 MAPK inhibitor affected bacterial replication, decreased phosphorylated p38 MAPK levels and increased tick cell death. However, XA reversed these effects. Taken together, we provide evidence that rickettsial pathogen modulates arthropod tryptophan and p38 MAPK pathways to inhibit cell death for its survival in ticks. Elsevier 2022-12-05 /pmc/articles/PMC9792911/ /pubmed/36582833 http://dx.doi.org/10.1016/j.isci.2022.105730 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Namjoshi, Prachi
Dahmani, Mustapha
Sultana, Hameeda
Neelakanta, Girish
Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title_full Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title_fullStr Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title_full_unstemmed Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title_short Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase
title_sort rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 map kinase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792911/
https://www.ncbi.nlm.nih.gov/pubmed/36582833
http://dx.doi.org/10.1016/j.isci.2022.105730
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