Chemically defined generation of human definitive hematopoietic stem and progenitor cells

Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endoth...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Juhyung, Chang, Yun, Jin, Gyuhyung, Deng, Qing, Lian, Xiaojun Lance, Bao, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792952/
https://www.ncbi.nlm.nih.gov/pubmed/36527716
http://dx.doi.org/10.1016/j.xpro.2022.101953
Descripción
Sumario:Here, we present a protocol to efficiently direct human pluripotent stem cells (hPSCs) into hematopoietic stem and progenitor cells (HSPCs) under a chemically defined, albumin-free system. We describe the induction of aorta-gonad-mesonephros-like hematopoiesis from hPSCs into SOX17+ hemogenic endothelium and then into CD34+CD45+ HSPCs via application of Wnt activator and TGFβ inhibitor, respectively. The generated HSPCs, characterized by flow cytometry and colony-forming unit assay, express definitive hematopoiesis markers and exhibit multilineage differentiation potential and the capacity to expand. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022a, 2022b).(1)(,)(2)

Ejemplares similares