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Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus

B cells, follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells are part of a circuit that may play a role in the development or progression of rheumatoid arthritis (RA). With the aim of providing further insight into this topic, here we evaluated the frequency of different subsets...

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Autores principales: Ferrero, Paola V., Onofrio, Luisina I., Acosta, Cristina del Valle, Zacca, Estefania R., Ponce, Nicolas E., Mussano, Eduardo, Onetti, Laura B., Cadile, Ignacio I., Costantino, Alicia B., Werner, Marina L., Mas, Luciana A., Alvarellos, Teresita, Montes, Carolina L., Acosta Rodríguez, Eva V., Gruppi, Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793086/
https://www.ncbi.nlm.nih.gov/pubmed/36582249
http://dx.doi.org/10.3389/fimmu.2022.1000982
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author Ferrero, Paola V.
Onofrio, Luisina I.
Acosta, Cristina del Valle
Zacca, Estefania R.
Ponce, Nicolas E.
Mussano, Eduardo
Onetti, Laura B.
Cadile, Ignacio I.
Costantino, Alicia B.
Werner, Marina L.
Mas, Luciana A.
Alvarellos, Teresita
Montes, Carolina L.
Acosta Rodríguez, Eva V.
Gruppi, Adriana
author_facet Ferrero, Paola V.
Onofrio, Luisina I.
Acosta, Cristina del Valle
Zacca, Estefania R.
Ponce, Nicolas E.
Mussano, Eduardo
Onetti, Laura B.
Cadile, Ignacio I.
Costantino, Alicia B.
Werner, Marina L.
Mas, Luciana A.
Alvarellos, Teresita
Montes, Carolina L.
Acosta Rodríguez, Eva V.
Gruppi, Adriana
author_sort Ferrero, Paola V.
collection PubMed
description B cells, follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells are part of a circuit that may play a role in the development or progression of rheumatoid arthritis (RA). With the aim of providing further insight into this topic, here we evaluated the frequency of different subsets of Tfh and Tfr in untreated and long-term treated RA patients from a cohort of Argentina, and their potential association with particular human leukocyte antigen (HLA) class-II variants and disease activity. We observed that the frequency of total Tfh cells as well as of particular Tfh subsets and Tfr cells were increased in seropositive untreated RA patients. Interestingly, when analyzing paired samples, the frequency of Tfh cells was reduced in synovial fluid compared to peripheral blood, while Tfr cells levels were similar in both biological fluids. After treatment, a decrease in the CCR7(lo)PD1(hi) Tfh subset and an increase in the frequency of Tfr cells was observed in blood. In comparison to healthy donors, seropositive patients with moderate and high disease activity exhibited higher frequency of Tfh cells while seropositive patients with low disease activity presented higher Tfr cell frequency. Finally, we observed that HLA-DRB1*09 presence correlated with higher frequency of Tfh and Tfr cells, while HLA-DRB1*04 was associated with increased Tfr cell frequency. Together, our results increase our knowledge about the dynamics of Tfh and Tfr cell subsets in RA, showing that this is altered after treatment.
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spelling pubmed-97930862022-12-28 Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus Ferrero, Paola V. Onofrio, Luisina I. Acosta, Cristina del Valle Zacca, Estefania R. Ponce, Nicolas E. Mussano, Eduardo Onetti, Laura B. Cadile, Ignacio I. Costantino, Alicia B. Werner, Marina L. Mas, Luciana A. Alvarellos, Teresita Montes, Carolina L. Acosta Rodríguez, Eva V. Gruppi, Adriana Front Immunol Immunology B cells, follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells are part of a circuit that may play a role in the development or progression of rheumatoid arthritis (RA). With the aim of providing further insight into this topic, here we evaluated the frequency of different subsets of Tfh and Tfr in untreated and long-term treated RA patients from a cohort of Argentina, and their potential association with particular human leukocyte antigen (HLA) class-II variants and disease activity. We observed that the frequency of total Tfh cells as well as of particular Tfh subsets and Tfr cells were increased in seropositive untreated RA patients. Interestingly, when analyzing paired samples, the frequency of Tfh cells was reduced in synovial fluid compared to peripheral blood, while Tfr cells levels were similar in both biological fluids. After treatment, a decrease in the CCR7(lo)PD1(hi) Tfh subset and an increase in the frequency of Tfr cells was observed in blood. In comparison to healthy donors, seropositive patients with moderate and high disease activity exhibited higher frequency of Tfh cells while seropositive patients with low disease activity presented higher Tfr cell frequency. Finally, we observed that HLA-DRB1*09 presence correlated with higher frequency of Tfh and Tfr cells, while HLA-DRB1*04 was associated with increased Tfr cell frequency. Together, our results increase our knowledge about the dynamics of Tfh and Tfr cell subsets in RA, showing that this is altered after treatment. Frontiers Media S.A. 2022-12-13 /pmc/articles/PMC9793086/ /pubmed/36582249 http://dx.doi.org/10.3389/fimmu.2022.1000982 Text en Copyright © 2022 Ferrero, Onofrio, Acosta, Zacca, Ponce, Mussano, Onetti, Cadile, Costantino, Werner, Mas, Alvarellos, Montes, Acosta Rodríguez and Gruppi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ferrero, Paola V.
Onofrio, Luisina I.
Acosta, Cristina del Valle
Zacca, Estefania R.
Ponce, Nicolas E.
Mussano, Eduardo
Onetti, Laura B.
Cadile, Ignacio I.
Costantino, Alicia B.
Werner, Marina L.
Mas, Luciana A.
Alvarellos, Teresita
Montes, Carolina L.
Acosta Rodríguez, Eva V.
Gruppi, Adriana
Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title_full Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title_fullStr Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title_full_unstemmed Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title_short Dynamics of circulating follicular helper T cell subsets and follicular regulatory T cells in rheumatoid arthritis patients according to HLA-DRB1 locus
title_sort dynamics of circulating follicular helper t cell subsets and follicular regulatory t cells in rheumatoid arthritis patients according to hla-drb1 locus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793086/
https://www.ncbi.nlm.nih.gov/pubmed/36582249
http://dx.doi.org/10.3389/fimmu.2022.1000982
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