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Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis

The spleen is a key immune-related organ that plays a role in communication between the brain and the immune system through the brain–spleen axis and brain–gut–microbiota axis. However, how the gut microbiota affects spleen and brain function remains unclear. Here, we investigated whether microbiome...

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Detalles Bibliográficos
Autores principales: Wan, Xiayun, Eguchi, Akifumi, Sakamoto, Akemi, Fujita, Yuko, Yang, Yong, Qu, Youge, Hatano, Masahiko, Mori, Chisato, Hashimoto, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793168/
https://www.ncbi.nlm.nih.gov/pubmed/36583066
http://dx.doi.org/10.1016/j.bbih.2022.100573
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author Wan, Xiayun
Eguchi, Akifumi
Sakamoto, Akemi
Fujita, Yuko
Yang, Yong
Qu, Youge
Hatano, Masahiko
Mori, Chisato
Hashimoto, Kenji
author_facet Wan, Xiayun
Eguchi, Akifumi
Sakamoto, Akemi
Fujita, Yuko
Yang, Yong
Qu, Youge
Hatano, Masahiko
Mori, Chisato
Hashimoto, Kenji
author_sort Wan, Xiayun
collection PubMed
description The spleen is a key immune-related organ that plays a role in communication between the brain and the immune system through the brain–spleen axis and brain–gut–microbiota axis. However, how the gut microbiota affects spleen and brain function remains unclear. Here, we investigated whether microbiome depletion induced by administration of an antibiotic cocktail (ABX) affects spleen and brain function. Treatment with ABX for 14 days resulted in a significant decrease in spleen weight and significant alterations in splenic functions, including the percentage of neutrophils, NK cells, macrophages, and CD8(+) T cells. Furthermore, ABX treatment resulted in the depletion of a large portion of the gut microbiota. Untargeted metabolomics analysis showed that ABX treatment caused alterations in the levels of certain compounds in the plasma, spleen, and brain. Moreover, ABX treatment decreased the expression of microglia marker Iba1 in the cerebral cortex. Interestingly, correlations were found between the abundance of different microbiome components and metabolites in various tissues, as well as splenic cell populations and spleen weight. These findings suggest that ABX-induced microbiome depletion and altered metabolite levels may affect spleen and brain function through the gut–microbiota–spleen–brain axis.
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spelling pubmed-97931682022-12-28 Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis Wan, Xiayun Eguchi, Akifumi Sakamoto, Akemi Fujita, Yuko Yang, Yong Qu, Youge Hatano, Masahiko Mori, Chisato Hashimoto, Kenji Brain Behav Immun Health Full Length Article The spleen is a key immune-related organ that plays a role in communication between the brain and the immune system through the brain–spleen axis and brain–gut–microbiota axis. However, how the gut microbiota affects spleen and brain function remains unclear. Here, we investigated whether microbiome depletion induced by administration of an antibiotic cocktail (ABX) affects spleen and brain function. Treatment with ABX for 14 days resulted in a significant decrease in spleen weight and significant alterations in splenic functions, including the percentage of neutrophils, NK cells, macrophages, and CD8(+) T cells. Furthermore, ABX treatment resulted in the depletion of a large portion of the gut microbiota. Untargeted metabolomics analysis showed that ABX treatment caused alterations in the levels of certain compounds in the plasma, spleen, and brain. Moreover, ABX treatment decreased the expression of microglia marker Iba1 in the cerebral cortex. Interestingly, correlations were found between the abundance of different microbiome components and metabolites in various tissues, as well as splenic cell populations and spleen weight. These findings suggest that ABX-induced microbiome depletion and altered metabolite levels may affect spleen and brain function through the gut–microbiota–spleen–brain axis. Elsevier 2022-12-17 /pmc/articles/PMC9793168/ /pubmed/36583066 http://dx.doi.org/10.1016/j.bbih.2022.100573 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Wan, Xiayun
Eguchi, Akifumi
Sakamoto, Akemi
Fujita, Yuko
Yang, Yong
Qu, Youge
Hatano, Masahiko
Mori, Chisato
Hashimoto, Kenji
Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title_full Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title_fullStr Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title_full_unstemmed Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title_short Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
title_sort impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793168/
https://www.ncbi.nlm.nih.gov/pubmed/36583066
http://dx.doi.org/10.1016/j.bbih.2022.100573
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