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Protocol for wireless deep brain stimulation in freely behaving mice with infrared light

Here, we present a protocol for deep brain stimulation in freely behaving mice using through-scalp wide-field illumination in the second near-infrared window (NIR-II). We first describe the injection of the TRPV1 (transient receptor potential cation channel subfamily V member 1)-expressing viruses a...

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Detalles Bibliográficos
Autores principales: Wu, Xiang, Hong, Guosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793315/
https://www.ncbi.nlm.nih.gov/pubmed/36538396
http://dx.doi.org/10.1016/j.xpro.2022.101757
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author Wu, Xiang
Hong, Guosong
author_facet Wu, Xiang
Hong, Guosong
author_sort Wu, Xiang
collection PubMed
description Here, we present a protocol for deep brain stimulation in freely behaving mice using through-scalp wide-field illumination in the second near-infrared window (NIR-II). We first describe the injection of the TRPV1 (transient receptor potential cation channel subfamily V member 1)-expressing viruses and macromolecular infrared nanotransducers for deep brain stimulation (MINDS). We then detail NIR-II neuromodulation in a conditioned place preference test, followed by immunohistochemical studies. This approach is especially useful for tether-free deep brain stimulation in social interacting experiments involving multiple subjects. For complete details on the use and execution of this protocol, please refer to Wu et al. (2022).
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spelling pubmed-97933152022-12-28 Protocol for wireless deep brain stimulation in freely behaving mice with infrared light Wu, Xiang Hong, Guosong STAR Protoc Protocol Here, we present a protocol for deep brain stimulation in freely behaving mice using through-scalp wide-field illumination in the second near-infrared window (NIR-II). We first describe the injection of the TRPV1 (transient receptor potential cation channel subfamily V member 1)-expressing viruses and macromolecular infrared nanotransducers for deep brain stimulation (MINDS). We then detail NIR-II neuromodulation in a conditioned place preference test, followed by immunohistochemical studies. This approach is especially useful for tether-free deep brain stimulation in social interacting experiments involving multiple subjects. For complete details on the use and execution of this protocol, please refer to Wu et al. (2022). Elsevier 2022-12-19 /pmc/articles/PMC9793315/ /pubmed/36538396 http://dx.doi.org/10.1016/j.xpro.2022.101757 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wu, Xiang
Hong, Guosong
Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title_full Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title_fullStr Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title_full_unstemmed Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title_short Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
title_sort protocol for wireless deep brain stimulation in freely behaving mice with infrared light
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793315/
https://www.ncbi.nlm.nih.gov/pubmed/36538396
http://dx.doi.org/10.1016/j.xpro.2022.101757
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