A competition smFRET assay to study ligand‐induced conformational changes of the dengue virus protease

Ligand binding to proteins often is accompanied by conformational transitions. Here, we describe a competition assay based on single molecule Förster resonance energy transfer (smFRET) to investigate the ligand‐induced conformational changes of the dengue virus (DENV) NS2B‐NS3 protease, which can ad...

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Detalles Bibliográficos
Autores principales: Maus, Hannah, Hinze, Gerald, Hammerschmidt, Stefan Josef, Basché, Thomas, Schirmeister, Tanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Full‐length Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793963/
https://www.ncbi.nlm.nih.gov/pubmed/36461913
http://dx.doi.org/10.1002/pro.4526

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9793963/
https://www.ncbi.nlm.nih.gov/pubmed/36461913
http://dx.doi.org/10.1002/pro.4526

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