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MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria

The extensive use of tetracycline antibiotics has led to the widespread presence of tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats to human and animal health. In our previous study, we reported a method for rapid detection of Tet(X)-producers using MALDI-TOF M...

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Autores principales: Zheng, Zi-Jian, Cui, Ze-Hua, Diao, Qiu-Yue, Ye, Xin-Qing, Zhong, Zi-Xing, Tang, Tian, Wu, Shuai-Bin, He, Hui-Ling, Lian, Xin-Lei, Fang, Liang-Xing, Wang, Xi-Ran, Liang, Li-Jie, Liu, Ya-Hong, Liao, Xiao-Ping, Sun, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794003/
https://www.ncbi.nlm.nih.gov/pubmed/34951562
http://dx.doi.org/10.1080/21505594.2021.2018768
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author Zheng, Zi-Jian
Cui, Ze-Hua
Diao, Qiu-Yue
Ye, Xin-Qing
Zhong, Zi-Xing
Tang, Tian
Wu, Shuai-Bin
He, Hui-Ling
Lian, Xin-Lei
Fang, Liang-Xing
Wang, Xi-Ran
Liang, Li-Jie
Liu, Ya-Hong
Liao, Xiao-Ping
Sun, Jian
author_facet Zheng, Zi-Jian
Cui, Ze-Hua
Diao, Qiu-Yue
Ye, Xin-Qing
Zhong, Zi-Xing
Tang, Tian
Wu, Shuai-Bin
He, Hui-Ling
Lian, Xin-Lei
Fang, Liang-Xing
Wang, Xi-Ran
Liang, Li-Jie
Liu, Ya-Hong
Liao, Xiao-Ping
Sun, Jian
author_sort Zheng, Zi-Jian
collection PubMed
description The extensive use of tetracycline antibiotics has led to the widespread presence of tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats to human and animal health. In our previous study, we reported a method for rapid detection of Tet(X)-producers using MALDI-TOF MS. However, there have been multiple machineries involved in tetracycline resistance including efflux pump, and ribosomal protection protein. Our previous demonstrated the limitation in probing the non-Tet(X)-producing tetracycline-resistant strains. In this regard, we further developed a MALDI-TOF MS method to detect and differentiate Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant strains. Test strains were incubated with tigecycline and oxytetracycline in separate tubes for 3 h and then analyzed spectral peaks of tigecycline, oxytetracycline, and their metabolite. Strains were distinguished using MS ratio for [metabolite/(metabolite+ tigecycline or oxytetracycline)]. Four control strains and 319 test strains were analyzed and the sensitivity was 98.90% and specificity was 98.34%. This was consistent with the results obtained from LC-MS/MS analysis. Interestingly, we also found that the reactive oxygen species (ROS) produced by tetracycline-susceptible strains were able to promote the degradation of oxytetracycline. Overall, the MALDI(Tet(X)-plus) test represents a rapid and reliable method to detect Tet(X)-producers, non-Tet(X)-producing tetracycline-resistant strains, and tetracycline-susceptible strains.
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spelling pubmed-97940032022-12-28 MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria Zheng, Zi-Jian Cui, Ze-Hua Diao, Qiu-Yue Ye, Xin-Qing Zhong, Zi-Xing Tang, Tian Wu, Shuai-Bin He, Hui-Ling Lian, Xin-Lei Fang, Liang-Xing Wang, Xi-Ran Liang, Li-Jie Liu, Ya-Hong Liao, Xiao-Ping Sun, Jian Virulence Research Paper The extensive use of tetracycline antibiotics has led to the widespread presence of tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats to human and animal health. In our previous study, we reported a method for rapid detection of Tet(X)-producers using MALDI-TOF MS. However, there have been multiple machineries involved in tetracycline resistance including efflux pump, and ribosomal protection protein. Our previous demonstrated the limitation in probing the non-Tet(X)-producing tetracycline-resistant strains. In this regard, we further developed a MALDI-TOF MS method to detect and differentiate Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant strains. Test strains were incubated with tigecycline and oxytetracycline in separate tubes for 3 h and then analyzed spectral peaks of tigecycline, oxytetracycline, and their metabolite. Strains were distinguished using MS ratio for [metabolite/(metabolite+ tigecycline or oxytetracycline)]. Four control strains and 319 test strains were analyzed and the sensitivity was 98.90% and specificity was 98.34%. This was consistent with the results obtained from LC-MS/MS analysis. Interestingly, we also found that the reactive oxygen species (ROS) produced by tetracycline-susceptible strains were able to promote the degradation of oxytetracycline. Overall, the MALDI(Tet(X)-plus) test represents a rapid and reliable method to detect Tet(X)-producers, non-Tet(X)-producing tetracycline-resistant strains, and tetracycline-susceptible strains. Taylor & Francis 2021-12-24 /pmc/articles/PMC9794003/ /pubmed/34951562 http://dx.doi.org/10.1080/21505594.2021.2018768 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zheng, Zi-Jian
Cui, Ze-Hua
Diao, Qiu-Yue
Ye, Xin-Qing
Zhong, Zi-Xing
Tang, Tian
Wu, Shuai-Bin
He, Hui-Ling
Lian, Xin-Lei
Fang, Liang-Xing
Wang, Xi-Ran
Liang, Li-Jie
Liu, Ya-Hong
Liao, Xiao-Ping
Sun, Jian
MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title_full MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title_fullStr MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title_full_unstemmed MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title_short MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria
title_sort maldi-tof ms for rapid detection and differentiation between tet(x)-producers and non-tet(x)-producing tetracycline-resistant gram-negative bacteria
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794003/
https://www.ncbi.nlm.nih.gov/pubmed/34951562
http://dx.doi.org/10.1080/21505594.2021.2018768
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