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Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis
BACKGROUND: The World Health Organization (WHO) reported an outbreak of monkeypox virus (MPXV) in Western countries on May 12(th), 2022. In early October, WHO counted 68 900 cases in the world outside Africa. MPXV spreads all around the environment of infected patients through direct contact with le...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The British Infection Association. Published by Elsevier Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794402/ https://www.ncbi.nlm.nih.gov/pubmed/36584769 http://dx.doi.org/10.1016/j.jinf.2022.12.025 |
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author | Mellon, Guillaume Rubenstein, Emma Antoine, Meghann Ferré, Valentine Marie Gabassi, Audrey Molina, Jean-Michel Delaugerre, Constance LeGoff, Jérôme |
author_facet | Mellon, Guillaume Rubenstein, Emma Antoine, Meghann Ferré, Valentine Marie Gabassi, Audrey Molina, Jean-Michel Delaugerre, Constance LeGoff, Jérôme |
author_sort | Mellon, Guillaume |
collection | PubMed |
description | BACKGROUND: The World Health Organization (WHO) reported an outbreak of monkeypox virus (MPXV) in Western countries on May 12(th), 2022. In early October, WHO counted 68 900 cases in the world outside Africa. MPXV spreads all around the environment of infected patients through direct contact with lesions, body secretion, or liquids. Interrogations about MPXV spreading through respiratory secretions have been reported but appear bewildering. Thus, we investigated for virus identification in the air around infected patients to move forward with unresolved questions. METHODS: We collected air samples using the AerosolSense™ device in a dedicated room where monkeypox suspected patients were examined in our quaternary hospital's outpatient infectious disease clinic. Samples were analyzed with a MPXV PCR to determine the presence of viral DNA in the air. RESULTS: The study took place from July 26(th) to August 5(th), 2022. We obtained seven four-hours-bioaerosol samples during the study period. Over the seven sessions sampled, six air samples were positive with a median Ct value of 36 (min-max: 32.0 – 38.0). Forty patients were present during the investigation; 17 (43%) were diagnosed monkeypox positive; 13 clinically and four virologically with a median Ct of 21 (min-max: 18.0 – 35.0). During the session, where no patients were diagnosed with monkeypox, air collection was also MPXV negative. CONCLUSION: This investigation reports the presence of MPXV DNA in air samples collected in a room dedicated to monkeypox-infected patients' examination and testing. Thus, we highlight the importance of personal protective equipment worn by consulting patients and healthcare workers and surface decontamination to avoid infection transmission. |
format | Online Article Text |
id | pubmed-9794402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The British Infection Association. Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97944022022-12-29 Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis Mellon, Guillaume Rubenstein, Emma Antoine, Meghann Ferré, Valentine Marie Gabassi, Audrey Molina, Jean-Michel Delaugerre, Constance LeGoff, Jérôme J Infect Letter to the Editor BACKGROUND: The World Health Organization (WHO) reported an outbreak of monkeypox virus (MPXV) in Western countries on May 12(th), 2022. In early October, WHO counted 68 900 cases in the world outside Africa. MPXV spreads all around the environment of infected patients through direct contact with lesions, body secretion, or liquids. Interrogations about MPXV spreading through respiratory secretions have been reported but appear bewildering. Thus, we investigated for virus identification in the air around infected patients to move forward with unresolved questions. METHODS: We collected air samples using the AerosolSense™ device in a dedicated room where monkeypox suspected patients were examined in our quaternary hospital's outpatient infectious disease clinic. Samples were analyzed with a MPXV PCR to determine the presence of viral DNA in the air. RESULTS: The study took place from July 26(th) to August 5(th), 2022. We obtained seven four-hours-bioaerosol samples during the study period. Over the seven sessions sampled, six air samples were positive with a median Ct value of 36 (min-max: 32.0 – 38.0). Forty patients were present during the investigation; 17 (43%) were diagnosed monkeypox positive; 13 clinically and four virologically with a median Ct of 21 (min-max: 18.0 – 35.0). During the session, where no patients were diagnosed with monkeypox, air collection was also MPXV negative. CONCLUSION: This investigation reports the presence of MPXV DNA in air samples collected in a room dedicated to monkeypox-infected patients' examination and testing. Thus, we highlight the importance of personal protective equipment worn by consulting patients and healthcare workers and surface decontamination to avoid infection transmission. The British Infection Association. Published by Elsevier Ltd. 2023-03 2022-12-28 /pmc/articles/PMC9794402/ /pubmed/36584769 http://dx.doi.org/10.1016/j.jinf.2022.12.025 Text en © 2022 The British Infection Association. Published by Elsevier Ltd. All rights reserved. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active. |
spellingShingle | Letter to the Editor Mellon, Guillaume Rubenstein, Emma Antoine, Meghann Ferré, Valentine Marie Gabassi, Audrey Molina, Jean-Michel Delaugerre, Constance LeGoff, Jérôme Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title | Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title_full | Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title_fullStr | Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title_full_unstemmed | Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title_short | Air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
title_sort | air detection of monkeypox virus in a dedicated outpatient clinic room for monkeypox infection diagnosis |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794402/ https://www.ncbi.nlm.nih.gov/pubmed/36584769 http://dx.doi.org/10.1016/j.jinf.2022.12.025 |
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