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Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis

Altered expression of adhesion molecules in immune cells has been demonstrated in rheumatoid arthritis (RA). Carcinoembryonic–antigen–related cell–adhesion molecule 1 (CEACAM1) is an adhesion molecule that acts as a coinhibitory receptor in the immune system. We investigated the role of CEACAM1 in i...

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Autores principales: Matsumoto, Haruki, Fujita, Yuya, Onizawa, Michio, Saito, Kenji, Sumichika, Yuya, Yoshida, Shuhei, Temmoku, Jumpei, Matsuoka, Naoki, Yashiro-Furuya, Makiko, Asano, Tomoyuki, Sato, Shuzo, Suzuki, Eiji, Machida, Takeshi, Watanabe, Hiroshi, Migita, Kiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794574/
https://www.ncbi.nlm.nih.gov/pubmed/36591283
http://dx.doi.org/10.3389/fimmu.2022.978435
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author Matsumoto, Haruki
Fujita, Yuya
Onizawa, Michio
Saito, Kenji
Sumichika, Yuya
Yoshida, Shuhei
Temmoku, Jumpei
Matsuoka, Naoki
Yashiro-Furuya, Makiko
Asano, Tomoyuki
Sato, Shuzo
Suzuki, Eiji
Machida, Takeshi
Watanabe, Hiroshi
Migita, Kiyoshi
author_facet Matsumoto, Haruki
Fujita, Yuya
Onizawa, Michio
Saito, Kenji
Sumichika, Yuya
Yoshida, Shuhei
Temmoku, Jumpei
Matsuoka, Naoki
Yashiro-Furuya, Makiko
Asano, Tomoyuki
Sato, Shuzo
Suzuki, Eiji
Machida, Takeshi
Watanabe, Hiroshi
Migita, Kiyoshi
author_sort Matsumoto, Haruki
collection PubMed
description Altered expression of adhesion molecules in immune cells has been demonstrated in rheumatoid arthritis (RA). Carcinoembryonic–antigen–related cell–adhesion molecule 1 (CEACAM1) is an adhesion molecule that acts as a coinhibitory receptor in the immune system. We investigated the role of CEACAM1 in immune cell subsets of patients with RA. Peripheral blood was obtained from 37 patients with RA and 20 healthy controls (HC). The expression of CEACAM1 and T–cell immunoglobulin mucin domain molecule (TIM) –3 on peripheral blood mononuclear cells and neutrophils was analyzed by flow cytometry. Intracellular TIM–3 expression was analyzed using cellular lysates by Western blot analysis. Serum levels of soluble CEACAM1 (sCEACAM1) were estimated by an enzyme-linked immunosorbent assay. CEACAM1 expression was not detected in peripheral blood mononuclear cells, including in CD14(+) monocytes and CD3(+) lymphocytes isolated from patients with RA or HC. However, substantial cell–surface expression of CEACAM1 was detected in peripheral blood neutrophils, and it was significantly elevated in samples from patients with RA without remission compared to those in remission. There was no significant difference in serum levels of sCEACAM1 between patients with RA and HC. Cell-surface expression of TIM-3 was not detected in peripheral blood neutrophils from patients with RA or HC but was seen in CD14(+) monocytes. However, there was no significant difference in TIM–3 expression on monocytes between patients with RA and HC. Our data indicate that cell-surface expression of CEACAM1 on peripheral blood neutrophils are higher in patients with RA and that it is associated with rheumatoid inflammation. Further studies are needed to explore the potential role of CEACAM1 in rheumatoid inflammatory pathways.
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spelling pubmed-97945742022-12-29 Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis Matsumoto, Haruki Fujita, Yuya Onizawa, Michio Saito, Kenji Sumichika, Yuya Yoshida, Shuhei Temmoku, Jumpei Matsuoka, Naoki Yashiro-Furuya, Makiko Asano, Tomoyuki Sato, Shuzo Suzuki, Eiji Machida, Takeshi Watanabe, Hiroshi Migita, Kiyoshi Front Immunol Immunology Altered expression of adhesion molecules in immune cells has been demonstrated in rheumatoid arthritis (RA). Carcinoembryonic–antigen–related cell–adhesion molecule 1 (CEACAM1) is an adhesion molecule that acts as a coinhibitory receptor in the immune system. We investigated the role of CEACAM1 in immune cell subsets of patients with RA. Peripheral blood was obtained from 37 patients with RA and 20 healthy controls (HC). The expression of CEACAM1 and T–cell immunoglobulin mucin domain molecule (TIM) –3 on peripheral blood mononuclear cells and neutrophils was analyzed by flow cytometry. Intracellular TIM–3 expression was analyzed using cellular lysates by Western blot analysis. Serum levels of soluble CEACAM1 (sCEACAM1) were estimated by an enzyme-linked immunosorbent assay. CEACAM1 expression was not detected in peripheral blood mononuclear cells, including in CD14(+) monocytes and CD3(+) lymphocytes isolated from patients with RA or HC. However, substantial cell–surface expression of CEACAM1 was detected in peripheral blood neutrophils, and it was significantly elevated in samples from patients with RA without remission compared to those in remission. There was no significant difference in serum levels of sCEACAM1 between patients with RA and HC. Cell-surface expression of TIM-3 was not detected in peripheral blood neutrophils from patients with RA or HC but was seen in CD14(+) monocytes. However, there was no significant difference in TIM–3 expression on monocytes between patients with RA and HC. Our data indicate that cell-surface expression of CEACAM1 on peripheral blood neutrophils are higher in patients with RA and that it is associated with rheumatoid inflammation. Further studies are needed to explore the potential role of CEACAM1 in rheumatoid inflammatory pathways. Frontiers Media S.A. 2022-12-14 /pmc/articles/PMC9794574/ /pubmed/36591283 http://dx.doi.org/10.3389/fimmu.2022.978435 Text en Copyright © 2022 Matsumoto, Fujita, Onizawa, Saito, Sumichika, Yoshida, Temmoku, Matsuoka, Yashiro-Furuya, Asano, Sato, Suzuki, Machida, Watanabe and Migita https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Matsumoto, Haruki
Fujita, Yuya
Onizawa, Michio
Saito, Kenji
Sumichika, Yuya
Yoshida, Shuhei
Temmoku, Jumpei
Matsuoka, Naoki
Yashiro-Furuya, Makiko
Asano, Tomoyuki
Sato, Shuzo
Suzuki, Eiji
Machida, Takeshi
Watanabe, Hiroshi
Migita, Kiyoshi
Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title_full Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title_fullStr Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title_full_unstemmed Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title_short Increased CEACAM1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
title_sort increased ceacam1 expression on peripheral blood neutrophils in patients with rheumatoid arthritis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794574/
https://www.ncbi.nlm.nih.gov/pubmed/36591283
http://dx.doi.org/10.3389/fimmu.2022.978435
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