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Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode

Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded...

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Autores principales: Nie, Ziyan, Li, Wenhua, Deng, Lili, Gao, Kai, Liu, Qinghua, Zhou, Zhichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795191/
https://www.ncbi.nlm.nih.gov/pubmed/36589100
http://dx.doi.org/10.3389/fpls.2022.1043261
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author Nie, Ziyan
Li, Wenhua
Deng, Lili
Gao, Kai
Liu, Qinghua
Zhou, Zhichun
author_facet Nie, Ziyan
Li, Wenhua
Deng, Lili
Gao, Kai
Liu, Qinghua
Zhou, Zhichun
author_sort Nie, Ziyan
collection PubMed
description Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded by leucine-rich repeat-containing transmembrane-receptor proteins play important roles in plant defense. Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest subfamily of the RLK protein family, play an important role in sensing stress signals in plants. However, the LRR-RLKs of P. massoniana have not been characterized previously, and their role in resistance to B. xylophilus is unknown. In this study, 185 members of the LRR-RLK subfamily were identified in P. massoniana and were categorized into 14 subgroups. Transcriptomic and quantitative real-time RT-PCR analyses showed that PmRLKs32 was highly expressed in the stem tissue after inoculation with B. xylophilus. The gene exhibited high homology with AtFLS2 of Arabidopsis thaliana. PmRLKs32 was localized to the plasma membrane and was significantly upregulated in nematode-resistant and nematode-susceptible individuals. The transient expression of PmRLKs32 resulted in a burst of reactive oxygen species production in P. massoniana and Nicotiana benthamiana seedlings. These results lay a foundation for further exploration of the regulatory mechanism of LRR-RLKs in response to biotic stress in P. massoniana.
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spelling pubmed-97951912022-12-29 Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode Nie, Ziyan Li, Wenhua Deng, Lili Gao, Kai Liu, Qinghua Zhou, Zhichun Front Plant Sci Plant Science Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded by leucine-rich repeat-containing transmembrane-receptor proteins play important roles in plant defense. Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest subfamily of the RLK protein family, play an important role in sensing stress signals in plants. However, the LRR-RLKs of P. massoniana have not been characterized previously, and their role in resistance to B. xylophilus is unknown. In this study, 185 members of the LRR-RLK subfamily were identified in P. massoniana and were categorized into 14 subgroups. Transcriptomic and quantitative real-time RT-PCR analyses showed that PmRLKs32 was highly expressed in the stem tissue after inoculation with B. xylophilus. The gene exhibited high homology with AtFLS2 of Arabidopsis thaliana. PmRLKs32 was localized to the plasma membrane and was significantly upregulated in nematode-resistant and nematode-susceptible individuals. The transient expression of PmRLKs32 resulted in a burst of reactive oxygen species production in P. massoniana and Nicotiana benthamiana seedlings. These results lay a foundation for further exploration of the regulatory mechanism of LRR-RLKs in response to biotic stress in P. massoniana. Frontiers Media S.A. 2022-12-14 /pmc/articles/PMC9795191/ /pubmed/36589100 http://dx.doi.org/10.3389/fpls.2022.1043261 Text en Copyright © 2022 Nie, Li, Deng, Gao, Liu and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Nie, Ziyan
Li, Wenhua
Deng, Lili
Gao, Kai
Liu, Qinghua
Zhou, Zhichun
Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title_full Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title_fullStr Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title_full_unstemmed Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title_short Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
title_sort comprehensive analysis of lrr-rlks and key gene identification in pinus massoniana resistant to pine wood nematode
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795191/
https://www.ncbi.nlm.nih.gov/pubmed/36589100
http://dx.doi.org/10.3389/fpls.2022.1043261
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