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Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode
Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795191/ https://www.ncbi.nlm.nih.gov/pubmed/36589100 http://dx.doi.org/10.3389/fpls.2022.1043261 |
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author | Nie, Ziyan Li, Wenhua Deng, Lili Gao, Kai Liu, Qinghua Zhou, Zhichun |
author_facet | Nie, Ziyan Li, Wenhua Deng, Lili Gao, Kai Liu, Qinghua Zhou, Zhichun |
author_sort | Nie, Ziyan |
collection | PubMed |
description | Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded by leucine-rich repeat-containing transmembrane-receptor proteins play important roles in plant defense. Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest subfamily of the RLK protein family, play an important role in sensing stress signals in plants. However, the LRR-RLKs of P. massoniana have not been characterized previously, and their role in resistance to B. xylophilus is unknown. In this study, 185 members of the LRR-RLK subfamily were identified in P. massoniana and were categorized into 14 subgroups. Transcriptomic and quantitative real-time RT-PCR analyses showed that PmRLKs32 was highly expressed in the stem tissue after inoculation with B. xylophilus. The gene exhibited high homology with AtFLS2 of Arabidopsis thaliana. PmRLKs32 was localized to the plasma membrane and was significantly upregulated in nematode-resistant and nematode-susceptible individuals. The transient expression of PmRLKs32 resulted in a burst of reactive oxygen species production in P. massoniana and Nicotiana benthamiana seedlings. These results lay a foundation for further exploration of the regulatory mechanism of LRR-RLKs in response to biotic stress in P. massoniana. |
format | Online Article Text |
id | pubmed-9795191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97951912022-12-29 Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode Nie, Ziyan Li, Wenhua Deng, Lili Gao, Kai Liu, Qinghua Zhou, Zhichun Front Plant Sci Plant Science Pinus massoniana is a pioneer tree widely planted for afforestation on barren hills in southern China where the total planted area is 8.04 million ha. The invasive pine wood nematode (Bursaphelenchus xylophilus) poses a serious threat to the survival of P. massoniana. Plant resistance genes encoded by leucine-rich repeat-containing transmembrane-receptor proteins play important roles in plant defense. Leucine-rich repeat receptor-like kinases (LRR-RLKs), the largest subfamily of the RLK protein family, play an important role in sensing stress signals in plants. However, the LRR-RLKs of P. massoniana have not been characterized previously, and their role in resistance to B. xylophilus is unknown. In this study, 185 members of the LRR-RLK subfamily were identified in P. massoniana and were categorized into 14 subgroups. Transcriptomic and quantitative real-time RT-PCR analyses showed that PmRLKs32 was highly expressed in the stem tissue after inoculation with B. xylophilus. The gene exhibited high homology with AtFLS2 of Arabidopsis thaliana. PmRLKs32 was localized to the plasma membrane and was significantly upregulated in nematode-resistant and nematode-susceptible individuals. The transient expression of PmRLKs32 resulted in a burst of reactive oxygen species production in P. massoniana and Nicotiana benthamiana seedlings. These results lay a foundation for further exploration of the regulatory mechanism of LRR-RLKs in response to biotic stress in P. massoniana. Frontiers Media S.A. 2022-12-14 /pmc/articles/PMC9795191/ /pubmed/36589100 http://dx.doi.org/10.3389/fpls.2022.1043261 Text en Copyright © 2022 Nie, Li, Deng, Gao, Liu and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Nie, Ziyan Li, Wenhua Deng, Lili Gao, Kai Liu, Qinghua Zhou, Zhichun Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title | Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title_full | Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title_fullStr | Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title_full_unstemmed | Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title_short | Comprehensive analysis of LRR-RLKs and key gene identification in Pinus massoniana resistant to pine wood nematode |
title_sort | comprehensive analysis of lrr-rlks and key gene identification in pinus massoniana resistant to pine wood nematode |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795191/ https://www.ncbi.nlm.nih.gov/pubmed/36589100 http://dx.doi.org/10.3389/fpls.2022.1043261 |
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