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Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy
PURPOSE: Study of the effects and mechanisms of licorice in the treatment of ulcerative colitis (UC) from the perspective of mitochondrial autophagy. METHODS: BALB/C mice were induced with 3% dextran sodium sulfate to build an animal model of UC. After 7 days of modeling, different doses of licorice...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795328/ https://www.ncbi.nlm.nih.gov/pubmed/36705402 http://dx.doi.org/10.1002/iid3.757 |
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author | Kong, Jinrong Xiang, Qingzhen Shi, Gaoxiang Xu, Zaiping Ma, Xiaowen Wang, Yunlai Xuan, Zihua Xu, Fan |
author_facet | Kong, Jinrong Xiang, Qingzhen Shi, Gaoxiang Xu, Zaiping Ma, Xiaowen Wang, Yunlai Xuan, Zihua Xu, Fan |
author_sort | Kong, Jinrong |
collection | PubMed |
description | PURPOSE: Study of the effects and mechanisms of licorice in the treatment of ulcerative colitis (UC) from the perspective of mitochondrial autophagy. METHODS: BALB/C mice were induced with 3% dextran sodium sulfate to build an animal model of UC. After 7 days of modeling, different doses of licorice were administered for 7 days. Hematoxylin and eosin staining is used to detect pathological changes in the colon. Mitochondrial membrane potentials and reactive oxygen species (ROS) contents were detected by flow cytometry, and autophagy of mitochondria was observed by transmission electron microscopy. Determination of inflammatory cytokines by enzyme‐linked immunosorbent assay. The oxidizing factors are detected by the kits. Western blot analysis was used to detect expressions for nuclear factor called erythropoietin (Nrf2), pten‐induced protein kinase 1 (PINK1), Parkin, HO‐1, P62, and LC3. RESULTS: Licorice improved the pathological condition of UC mice, increasing the mitochondrial membrane potential and decreasing the ROS content. Promotes the emergence of autophagosomes and autophagosomes. The contents of interleukin (IL)‐1β, IL‐6, IL‐17, and tumor necrosis factor‐alpha were downregulated, the contents of superoxide dismutase and glutathione peroxidase were upregulated and the contents of malondialdehyde were downregulated. In addition, licorice promotes the expression of Nrf2, PINK1, Parkin, HO‐1, P62, and LC3. CONCLUSION: Licorice was shown to reduce levels of inflammatory factors and oxidative stress in mice with UC, possibly by promoting mitochondrial autophagy through the activation of the Nrf2/PINK1 pathway. |
format | Online Article Text |
id | pubmed-9795328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97953282022-12-30 Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy Kong, Jinrong Xiang, Qingzhen Shi, Gaoxiang Xu, Zaiping Ma, Xiaowen Wang, Yunlai Xuan, Zihua Xu, Fan Immun Inflamm Dis Original Articles PURPOSE: Study of the effects and mechanisms of licorice in the treatment of ulcerative colitis (UC) from the perspective of mitochondrial autophagy. METHODS: BALB/C mice were induced with 3% dextran sodium sulfate to build an animal model of UC. After 7 days of modeling, different doses of licorice were administered for 7 days. Hematoxylin and eosin staining is used to detect pathological changes in the colon. Mitochondrial membrane potentials and reactive oxygen species (ROS) contents were detected by flow cytometry, and autophagy of mitochondria was observed by transmission electron microscopy. Determination of inflammatory cytokines by enzyme‐linked immunosorbent assay. The oxidizing factors are detected by the kits. Western blot analysis was used to detect expressions for nuclear factor called erythropoietin (Nrf2), pten‐induced protein kinase 1 (PINK1), Parkin, HO‐1, P62, and LC3. RESULTS: Licorice improved the pathological condition of UC mice, increasing the mitochondrial membrane potential and decreasing the ROS content. Promotes the emergence of autophagosomes and autophagosomes. The contents of interleukin (IL)‐1β, IL‐6, IL‐17, and tumor necrosis factor‐alpha were downregulated, the contents of superoxide dismutase and glutathione peroxidase were upregulated and the contents of malondialdehyde were downregulated. In addition, licorice promotes the expression of Nrf2, PINK1, Parkin, HO‐1, P62, and LC3. CONCLUSION: Licorice was shown to reduce levels of inflammatory factors and oxidative stress in mice with UC, possibly by promoting mitochondrial autophagy through the activation of the Nrf2/PINK1 pathway. John Wiley and Sons Inc. 2022-12-28 /pmc/articles/PMC9795328/ /pubmed/36705402 http://dx.doi.org/10.1002/iid3.757 Text en © 2022 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Kong, Jinrong Xiang, Qingzhen Shi, Gaoxiang Xu, Zaiping Ma, Xiaowen Wang, Yunlai Xuan, Zihua Xu, Fan Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title | Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title_full | Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title_fullStr | Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title_full_unstemmed | Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title_short | Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy |
title_sort | licorice protects against ulcerative colitis via the nrf2/pink1‐mediated mitochondrial autophagy |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795328/ https://www.ncbi.nlm.nih.gov/pubmed/36705402 http://dx.doi.org/10.1002/iid3.757 |
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