A culture platform to study quiescent hematopoietic stem cells following genome editing

Other than genetically engineered mice, few reliable platforms are available for the study of hematopoietic stem cell (HSC) quiescence. Here we present a platform to analyze HSC cell cycle quiescence by combining culture conditions that maintain quiescence with a CRISPR-Cas9 genome editing system op...

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Detalles Bibliográficos
Autores principales: Shiroshita, Kohei, Kobayashi, Hiroshi, Watanuki, Shintaro, Karigane, Daiki, Sorimachi, Yuriko, Fujita, Shinya, Tamaki, Shinpei, Haraguchi, Miho, Itokawa, Naoki, Aoyoama, Kazumasa, Koide, Shuhei, Masamoto, Yosuke, Kobayashi, Kenta, Nakamura-Ishizu, Ayako, Kurokawa, Mineo, Iwama, Atsushi, Okamoto, Shinichiro, Kataoka, Keisuke, Takubo, Keiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795334/
https://www.ncbi.nlm.nih.gov/pubmed/36590688
http://dx.doi.org/10.1016/j.crmeth.2022.100354

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