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In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior collic...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795524/ https://www.ncbi.nlm.nih.gov/pubmed/36542520 http://dx.doi.org/10.1016/j.xpro.2022.101963 |
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author | Broersen, Robin Stuart, Greg J. |
author_facet | Broersen, Robin Stuart, Greg J. |
author_sort | Broersen, Robin |
collection | PubMed |
description | In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas. |
format | Online Article Text |
id | pubmed-9795524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97955242022-12-29 In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons Broersen, Robin Stuart, Greg J. STAR Protoc Protocol In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas. Elsevier 2022-12-20 /pmc/articles/PMC9795524/ /pubmed/36542520 http://dx.doi.org/10.1016/j.xpro.2022.101963 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Broersen, Robin Stuart, Greg J. In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title | In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title_full | In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title_fullStr | In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title_full_unstemmed | In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title_short | In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
title_sort | in vivo whole-cell recording from morphologically identified mouse superior colliculus neurons |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795524/ https://www.ncbi.nlm.nih.gov/pubmed/36542520 http://dx.doi.org/10.1016/j.xpro.2022.101963 |
work_keys_str_mv | AT broersenrobin invivowholecellrecordingfrommorphologicallyidentifiedmousesuperiorcolliculusneurons AT stuartgregj invivowholecellrecordingfrommorphologicallyidentifiedmousesuperiorcolliculusneurons |