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In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons

In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior collic...

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Detalles Bibliográficos
Autores principales: Broersen, Robin, Stuart, Greg J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795524/
https://www.ncbi.nlm.nih.gov/pubmed/36542520
http://dx.doi.org/10.1016/j.xpro.2022.101963
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author Broersen, Robin
Stuart, Greg J.
author_facet Broersen, Robin
Stuart, Greg J.
author_sort Broersen, Robin
collection PubMed
description In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas.
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spelling pubmed-97955242022-12-29 In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons Broersen, Robin Stuart, Greg J. STAR Protoc Protocol In vivo whole-cell recording, when combined with morphological characterization after biocytin labeling, is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we describe steps for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. We detail two types of visual stimulation techniques: full-field light-emitting diode (LED) flashes and visual stimuli shown on monitors. While we focus on superior colliculus, this protocol is applicable to other brain areas. Elsevier 2022-12-20 /pmc/articles/PMC9795524/ /pubmed/36542520 http://dx.doi.org/10.1016/j.xpro.2022.101963 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Broersen, Robin
Stuart, Greg J.
In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title_full In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title_fullStr In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title_full_unstemmed In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title_short In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
title_sort in vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795524/
https://www.ncbi.nlm.nih.gov/pubmed/36542520
http://dx.doi.org/10.1016/j.xpro.2022.101963
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