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Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture
Liver disease is expanding across the globe; however, health‐care systems still lack approved pharmaceutical treatment strategies to mitigate potential liver failures. Organ transplantation is the only treatment for liver failure and with increasing cases of liver disease, transplant programs increa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796056/ https://www.ncbi.nlm.nih.gov/pubmed/35734943 http://dx.doi.org/10.1002/jbm.b.35115 |
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author | Bate, Thomas S. R. Shanahan, William Casillo, Joseph P. Grant, Rhiannon Forbes, Stuart J. Callanan, Anthony |
author_facet | Bate, Thomas S. R. Shanahan, William Casillo, Joseph P. Grant, Rhiannon Forbes, Stuart J. Callanan, Anthony |
author_sort | Bate, Thomas S. R. |
collection | PubMed |
description | Liver disease is expanding across the globe; however, health‐care systems still lack approved pharmaceutical treatment strategies to mitigate potential liver failures. Organ transplantation is the only treatment for liver failure and with increasing cases of liver disease, transplant programs increasingly cannot provide timely transplant availability for all patients. The development of pharmaceutical mitigation strategies is clearly necessary and methods to improve drug development processes are considered vital for this purpose. Herein, we present a methodology for incorporating whole organ decellularised rat liver ECM (rLECM) into polycaprolactone (PCL) electrospun scaffolds with the aim of producing biologically relevant liver tissue models. rLECM PCL scaffolds have been produced with 5 w/w% and 10 w/w% rLECM:PCL and were analyzed by SEM imaging, tensile mechanical analyses and FTIR spectroscopy. The hepatocellular carcinoma cell line, HepG2, was cultured upon the scaffolds for 14 days and were analyzed through cell viability assay, DNA quantification, albumin quantification, immunohistochemistry, and RT‐qPCR gene expression analysis. Results showed significant increases in proliferative activity of HepG2 on rLECM containing scaffolds alongside maintained key gene expression. This study confirms that rLECM can be utilized to modulate the bioactivity of electrospun PCL scaffolds and has the potential to produce electrospun scaffolds suitable for enhanced hepatocyte cultures and in‐vitro liver tissue models. |
format | Online Article Text |
id | pubmed-9796056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97960562022-12-28 Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture Bate, Thomas S. R. Shanahan, William Casillo, Joseph P. Grant, Rhiannon Forbes, Stuart J. Callanan, Anthony J Biomed Mater Res B Appl Biomater Research Articles Liver disease is expanding across the globe; however, health‐care systems still lack approved pharmaceutical treatment strategies to mitigate potential liver failures. Organ transplantation is the only treatment for liver failure and with increasing cases of liver disease, transplant programs increasingly cannot provide timely transplant availability for all patients. The development of pharmaceutical mitigation strategies is clearly necessary and methods to improve drug development processes are considered vital for this purpose. Herein, we present a methodology for incorporating whole organ decellularised rat liver ECM (rLECM) into polycaprolactone (PCL) electrospun scaffolds with the aim of producing biologically relevant liver tissue models. rLECM PCL scaffolds have been produced with 5 w/w% and 10 w/w% rLECM:PCL and were analyzed by SEM imaging, tensile mechanical analyses and FTIR spectroscopy. The hepatocellular carcinoma cell line, HepG2, was cultured upon the scaffolds for 14 days and were analyzed through cell viability assay, DNA quantification, albumin quantification, immunohistochemistry, and RT‐qPCR gene expression analysis. Results showed significant increases in proliferative activity of HepG2 on rLECM containing scaffolds alongside maintained key gene expression. This study confirms that rLECM can be utilized to modulate the bioactivity of electrospun PCL scaffolds and has the potential to produce electrospun scaffolds suitable for enhanced hepatocyte cultures and in‐vitro liver tissue models. John Wiley & Sons, Inc. 2022-06-23 2022-12 /pmc/articles/PMC9796056/ /pubmed/35734943 http://dx.doi.org/10.1002/jbm.b.35115 Text en © 2022 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Bate, Thomas S. R. Shanahan, William Casillo, Joseph P. Grant, Rhiannon Forbes, Stuart J. Callanan, Anthony Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title | Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title_full | Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title_fullStr | Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title_full_unstemmed | Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title_short | Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
title_sort | rat liver ecm incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796056/ https://www.ncbi.nlm.nih.gov/pubmed/35734943 http://dx.doi.org/10.1002/jbm.b.35115 |
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