Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma...

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Detalles Bibliográficos
Autores principales: Boodman, Carl, Loomer, Courtney, Dibernardo, Antonia, Hatchette, Todd, LeBlanc, Jason J., Waitt, Brooks, Lindsay, L. Robbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2023
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796190/
https://www.ncbi.nlm.nih.gov/pubmed/36573611
http://dx.doi.org/10.3201/eid2901.220988
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author Boodman, Carl
Loomer, Courtney
Dibernardo, Antonia
Hatchette, Todd
LeBlanc, Jason J.
Waitt, Brooks
Lindsay, L. Robbin
author_facet Boodman, Carl
Loomer, Courtney
Dibernardo, Antonia
Hatchette, Todd
LeBlanc, Jason J.
Waitt, Brooks
Lindsay, L. Robbin
author_sort Boodman, Carl
collection PubMed
description Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.
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spelling pubmed-97961902023-01-09 Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada Boodman, Carl Loomer, Courtney Dibernardo, Antonia Hatchette, Todd LeBlanc, Jason J. Waitt, Brooks Lindsay, L. Robbin Emerg Infect Dis Dispatch Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable. Centers for Disease Control and Prevention 2023-01 /pmc/articles/PMC9796190/ /pubmed/36573611 http://dx.doi.org/10.3201/eid2901.220988 Text en https://creativecommons.org/licenses/by/4.0/Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Boodman, Carl
Loomer, Courtney
Dibernardo, Antonia
Hatchette, Todd
LeBlanc, Jason J.
Waitt, Brooks
Lindsay, L. Robbin
Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title_full Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title_fullStr Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title_full_unstemmed Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title_short Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
title_sort using serum specimens for real-time pcr-based diagnosis of human granulocytic anaplasmosis, canada
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796190/
https://www.ncbi.nlm.nih.gov/pubmed/36573611
http://dx.doi.org/10.3201/eid2901.220988
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