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Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos

The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media....

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Autores principales: Martínez‐Rodero, Iris, Salas‐Huetos, Albert, Ordóñez‐León, Alina, Hidalgo, Carlos Olegario, Yeste, Marc, Mercadé, Elena, Mogas, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796228/
https://www.ncbi.nlm.nih.gov/pubmed/35748223
http://dx.doi.org/10.1111/rda.14191
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author Martínez‐Rodero, Iris
Salas‐Huetos, Albert
Ordóñez‐León, Alina
Hidalgo, Carlos Olegario
Yeste, Marc
Mercadé, Elena
Mogas, Teresa
author_facet Martínez‐Rodero, Iris
Salas‐Huetos, Albert
Ordóñez‐León, Alina
Hidalgo, Carlos Olegario
Yeste, Marc
Mercadé, Elena
Mogas, Teresa
author_sort Martínez‐Rodero, Iris
collection PubMed
description The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p < .05) and higher GPX1 (p < .05) and CDX2 (p < .1) gene expression were observed in expanded and/or hatched blastocysts derived from VitTrans‐EPS‐vitrified embryos when compared to those from the non‐supplemented group. In conclusion, addition of EPS not only promoted blastocyst survival and hatching after VitTrans vitrification/warming but also modified the expression of genes associated with better embryo quality.
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spelling pubmed-97962282022-12-30 Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos Martínez‐Rodero, Iris Salas‐Huetos, Albert Ordóñez‐León, Alina Hidalgo, Carlos Olegario Yeste, Marc Mercadé, Elena Mogas, Teresa Reprod Domest Anim Short Communications The cold‐adapted bacterium Pseudomonas sp. ID1 produces the extracellular exopolysaccharide ID1 (EPS ID1) with cryoprotective activity. This study was designed to optimize the vitrification/in‐straw warming protocol of in vitro‐produced (IVP) blastocysts by adding EPS ID1 to the vitrification media. Day 7‐expanded blastocysts were vitrified/warmed using the VitTrans device after the addition of 0 or 100 μg/mL EPS ID1 to the vitrification media. Blastocysts vitrified by the Cryotop method and fresh non‐vitrified blastocysts served as controls. Outcomes were assessed in the warmed embryos in terms of survival rates and mRNA relative abundances of BAX, BCL2, GPX1, and CDX2 genes. No differences in survival rates were observed at 3 h post‐warming between vitrification treatments. At 24 h post‐warming, the addition of EPS prior to vitrification with the VitTrans device produced similar survival rates to Cryotop‐vitrified embryos and similar hatching rates to fresh non‐vitrified or Cryotop‐vitrified embryos. No differences emerged in BCL2 gene expression. Lower BAX (p < .05) and higher GPX1 (p < .05) and CDX2 (p < .1) gene expression were observed in expanded and/or hatched blastocysts derived from VitTrans‐EPS‐vitrified embryos when compared to those from the non‐supplemented group. In conclusion, addition of EPS not only promoted blastocyst survival and hatching after VitTrans vitrification/warming but also modified the expression of genes associated with better embryo quality. John Wiley and Sons Inc. 2022-07-12 2022-10 /pmc/articles/PMC9796228/ /pubmed/35748223 http://dx.doi.org/10.1111/rda.14191 Text en © 2022 The Authors. Reproduction in Domestic Animals published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Short Communications
Martínez‐Rodero, Iris
Salas‐Huetos, Albert
Ordóñez‐León, Alina
Hidalgo, Carlos Olegario
Yeste, Marc
Mercadé, Elena
Mogas, Teresa
Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title_full Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title_fullStr Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title_full_unstemmed Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title_short Cryoprotectant role of exopolysaccharide ID1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
title_sort cryoprotectant role of exopolysaccharide id1 in the vitrification/in‐straw warming of in vitro‐produced bovine embryos
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796228/
https://www.ncbi.nlm.nih.gov/pubmed/35748223
http://dx.doi.org/10.1111/rda.14191
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