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Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides

The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin‐mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investig...

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Autores principales: Chowdhury, Jamil, Kemppainen, Minna, Delhomme, Nicolas, Shutava, Iryna, Zhou, Jingjing, Takahashi, Junko, Pardo, Alejandro G., Lundberg‐Felten, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796311/
https://www.ncbi.nlm.nih.gov/pubmed/35794841
http://dx.doi.org/10.1111/nph.18358
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author Chowdhury, Jamil
Kemppainen, Minna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro G.
Lundberg‐Felten, Judith
author_facet Chowdhury, Jamil
Kemppainen, Minna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro G.
Lundberg‐Felten, Judith
author_sort Chowdhury, Jamil
collection PubMed
description The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin‐mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell‐wall‐related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de‐esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM‐induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de‐esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
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spelling pubmed-97963112022-12-30 Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides Chowdhury, Jamil Kemppainen, Minna Delhomme, Nicolas Shutava, Iryna Zhou, Jingjing Takahashi, Junko Pardo, Alejandro G. Lundberg‐Felten, Judith New Phytol Research The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin‐mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell‐wall‐related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de‐esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM‐induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de‐esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation. John Wiley and Sons Inc. 2022-07-27 2022-10 /pmc/articles/PMC9796311/ /pubmed/35794841 http://dx.doi.org/10.1111/nph.18358 Text en © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research
Chowdhury, Jamil
Kemppainen, Minna
Delhomme, Nicolas
Shutava, Iryna
Zhou, Jingjing
Takahashi, Junko
Pardo, Alejandro G.
Lundberg‐Felten, Judith
Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_full Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_fullStr Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_full_unstemmed Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_short Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
title_sort laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with populus tremula × populus tremuloides
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796311/
https://www.ncbi.nlm.nih.gov/pubmed/35794841
http://dx.doi.org/10.1111/nph.18358
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